SB 202190
Tocris Bioscience | Catalog # 1264
Product Description
SB 202190 is a highly selective, potent and cell-permeable inhibitor of p38 MAP kinase. Binds within the ATP pocket of the active kinase (Kd = 38 nM, as measured in recombinant human p38), and selectively inhibits the p38α and β isoforms (IC50 = 50 and 100 nM at SAPK2a/p38 and SAPK2b/p38β2 respectively). Promotes stability of naive human pluripotent stem cells in culture. Inhibits Yamanaka factor reprogramming of human fibroblasts to iPSCs. SB 202190 improves the self-renewal ability of neuronal stem cells from NPC1-deficient mice.Scientific Data Images for SB 202190
Application of SB 202190 in Culture and Characterization of iPSC-derived Human Intestinal Organoids.
iPSC-derived human intestinal organoids were cultured using SB 202190, Y-27632 dihydrochloride (Catalog # 1254), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).
Application of SB 202190 in Culture and Characterization of Adult Stem Cell-derived Lung Organoids.
Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes SB 202190, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Human Cytokeratin 5 (KRT5) Antibody (green) and a Human p63/TP73L Antibody (Catalog # AF1916; red) to visualize basal cells, (B) a Mouse Podoplanin (PDPN) Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Human p63/TP73L Antibody (Catalog # AF1916; red) to visualize basal cells, and (C, D) a MUC5AC Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Human/Mouse/Rat SOX2 Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).
Application of SB 202190 in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids.
Adult stem cells isolated from human descending colon were embedded and then cultured for 30 days in intestinal organoid culture medium, which includes SB 202190, Y-27632 dihydrochloride (Catalog # 1254), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Human MUC2 Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Human/Mouse E-Cadherin Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue).(B) Organoids were fixed and stained with a Human Chromogranin A Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Human/Mouse E-Cadherin Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue).
Licensing Information
Sold with the permission of GlaxoSmithKline
Product Specifications for SB 202190
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 33.13 | 100 |
Preparing Stock Solutions for SB 202190
The following data is based on the product molecular weight 331.35.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.02 mL | 15.09 mL | 30.18 mL |
| 5 mM | 0.60 mL | 3.02 mL | 6.04 mL |
| 10 mM | 0.30 mL | 1.51 mL | 3.02 mL |
| 50 mM | 0.06 mL | 0.30 mL | 0.60 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 342 publications citing the usage of this product.
- Sato Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology 2011 PMID: 21889923
- Yang NPC1 gene deficiency leads to lack of neural stem cell self-renewal and abnormal differentiation through activation of p38 mitogen-activated protein kinase signaling. Stem Cells 2006 PMID: 16099992
- Karthaus Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell 2014 PMID: 25201529
- Neganova A critical role for p38MAPK signalling pathway during reprogramming of human fibroblasts to iPSCs. Sci.Rep. 2017 PMID: 28155868
- Sato SnapShot: Growing Organoids from Stem Cells. Cell 2015 PMID: 26091044
- Bartfeld In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 2015 PMID: 25307862
- Gafni Derivation of novel human ground state naive pluripotent stem cells. Nature 2013 PMID: 24172903
- Nemoto Induction of apoptosis by SB 202190 through inhibition of p38β mitogen-activated protein kinase. J.Biol.Chem. 1998 PMID: 9632706
- Jiang Characterization of the structure and function of a new mitogen-activated protein kinase (p38β). J.Biol.Chem. 1996 PMID: 8663524
- Frantz The activation of p38 mitogen-activated protein kinase determines the efficiency of ATP competition for pyridinylimidazole inhibitor binding. Biochemistry 1998 PMID: 9753474
- Davies Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 2000 PMID: 10998351
Product Documents for SB 202190
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SB 202190
For research use only
Citations for SB 202190
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Species: HumanAssay Type: Ex VivoVerified Customer | Posted 02/07/2019I used this to test the potency of this to kill of COLO 205 cells with the addition of TNF in a dose dependent manner.
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Assay Type: In VitroCell Line/Tissue: MDA-MB-231Verified Customer | Posted 12/26/2018I compared this inhibitor to a TAK1 inhibitor to compared effects on cell death.
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