|Chemotaxis Induced by CXCL1/CINC‑1 and Neutralization by Rat CXCL1/CINC‑1 Antibody. Recombinant Rat CXCL1/CINC‑1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC‑1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND50 is typically 0.15-0.75 µg/mL.|
|CXCL1/GRO alpha /KC/CINC‑1 in Rat Liver. CXCL1/GRO alpha /KC/CINC‑1 was detected in perfusion fixed paraffin-embedded sections of rat liver using 15 µg/mL Rat CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑515‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
|CXCL1/GRO alpha /KC/CINC‑1 in Rat Liver. CXCL1/GRO alpha /KC/CINC‑1 was detected in perfusion fixed frozen sections of rat liver using Rat CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.|
Cytokine-induced neutrophil chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2 alpha, CINC-2 beta, CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha, GRO-beta, and GRO-gamma, respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs.
Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.
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