Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

(2 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant rat (rr) CXCL3 and less than 0.1% cross-reactivity with rrCXCL1, rrCINC-2 alpha, recombinant mouse (rm) CXCL9 or rmCXCL2 is observed. In Western blots, less than 3% cross-reactivity with recombinant human (rh) CXCL8, recombinant porcine CXCL8, or rrCINC-2 alpha and no cross‑reactivity with rrCXCL1, 3, rmCXCL1, 2, 6, 9, 10, 12, rhCXLC1, 2, 3, 5, 6, 7, 9,10, 11, 12 or 13 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 123802
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3
    Ser32-Asn100
    Accession # P30348
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 525-C3)
    • Rat CXCL2/CINC-3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog # MAB525)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antibody (Catalog # BAF525)
  • ELISA Standard
     
    Recombinant Rat CXCL2/CINC-3 Protein (Catalog # 525-C3)
  • Neutralization
    Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.1 µg/mL Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL2/CINC‑3 and Neutral­ization by Rat CXCL2/ CINC‑3 Antibody. Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/
CINC‑3 (0.1 µg/mL) is neutral­ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC‑3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4‑2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

  • References:
    1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
    2. Huang, S. et al. (1992) Am. J. Pathol. 141:981.
  • Entrez Gene IDs:
    2920 (Human); 20310 (Mouse); 114105 (Rat)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007;369(2):288-98.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  2. Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats.
    Authors: Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA
    J. Mol. Cell. Cardiol., 2006;41(2):296-307.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0042 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Antibody

Flow MAB0043  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information