Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant rat (rr) CXCL3 and less than 0.1% cross-reactivity with rrCXCL1, rrCINC-2 alpha, recombinant mouse (rm) CXCL9 or rmCXCL2 is observed. In Western blots, less than 3% cross-reactivity with recombinant human (rh) CXCL8, recombinant porcine CXCL8, or rrCINC-2 alpha and no cross‑reactivity with rrCXCL1, 3, rmCXCL1, 2, 6, 9, 10, 12, rhCXLC1, 2, 3, 5, 6, 7, 9,10, 11, 12 or 13 is observed.
Monoclonal Mouse IgG2B Clone # 123802
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3 Ser32-Asn100 Accession # P30348
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 525-C3)
Rat CXCL2/CINC-3 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog # MAB525)
ELISA Detection (Matched Antibody Pair)
Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antibody (Catalog # BAF525)
Recombinant Rat CXCL2/CINC-3 Protein (Catalog # 525-C3)
Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.1 µg/mL Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL2/CINC‑3 and Neutralization by Rat CXCL2/ CINC‑3 Antibody. Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/ CINC‑3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC‑3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4‑2.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.
CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.
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