Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody Summary
Accession # P30348
Rat CXCL2/CINC-3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL2/CINC‑3 and Neutral-ization by Rat CXCL2/ CINC‑3 Antibody. Recombinant Rat CXCL2/CINC-3 (Catalog # 525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/ CINC-3 (0.1 µg/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC-3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4-2.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.
CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.
- Nakagawa, H. et al. (1994) Biochem. J. 301:545.
- Huang, S. et al. (1992) Am. J. Pathol. 141:981.
Citations for Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody
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Citations: Showing 1 - 2
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Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Authors: Miura TA, Wang J, Holmes KV, Mason RJ
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats.
Authors: Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA
J. Mol. Cell. Cardiol., 2006;41(2):296-307.
Sample Types: Tissue Homogenates
Applications: ELISA Development
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