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Rat Growth Hormone Antibody

Catalog #: MAB1566
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1566
MAB1566-SP

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Detection of Rat Growth Hormone by Western Blot.
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Product Details
Citations (4)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Growth Hormone Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Growth Hormone in direct ELISAs and Western blots. Does not cross‑react with recombinant human Growth Hormone.
Source
Monoclonal Mouse IgG2B Clone # 222540
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Growth Hormone
Phe27-Phe216
Accession # P01244
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Growth Hormone antibody by Western Blot. View Larger

Detection of Rat Growth Hormone by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Growth Hormone Monoclonal Antibody (Catalog # MAB1566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Growth Hormone at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth Hormone, also known as somatotropin, is synthesized primarily in the anterior pituitary. It is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor which is present in many cell types.

Entrez Gene IDs
2688 (Human)
Alternate Names
GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin

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Citations for Rat Growth Hormone Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Somatostatin receptor subtype 5 modifies hypothalamic-pituitary-adrenal axis stress function
    Authors: Masaaki Yamamoto, Anat Ben-Shlomo, Hiraku Kameda, Hidenori Fukuoka, Nan Deng, Yan Ding et al.
    JCI Insight
  2. Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation
    Authors: Haojie Zhang, Qien Qi, Ting Chen, Junyi Luo, Qianyun Xi, Qingyan Jiang et al.
    International Journal of Molecular Sciences
  3. Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation
    Authors: Haojie Zhang, Qien Qi, Ting Chen, Junyi Luo, Qianyun Xi, Qingyan Jiang et al.
    International Journal of Molecular Sciences
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Databases for technical aspects of immunohistochemistry-2021 update
    Authors: MOROKI T, MATSUO S, HATAKEYAMA H et al.
    Journal of Toxicologic Pathology

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