Rat Growth Hormone R/GHR Antibody

Catalog # Availability Size / Price Qty
AF1211
AF1211-SP
Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody.
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Rat Growth Hormone R/GHR Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Growth Hormone R/GHR in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse GHR and recombinant human GHR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Growth Hormone R/GHR
Phe19-Arg265
Accession # P16310
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Growth Hormone R/GHR Fc Chimera (Catalog # 1211-GR)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of rat thymus
Neutralization
Measured by its ability to neutralize Growth Hormone R/GHR-mediated inhibition of proliferation in the Nb2‑11 rat lymphoma cell line. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 10 ng/mL Recombinant Rat Growth Hormone R/GHR Fc Chimera and 0.2 ng/mL Recombinant Human Growth Hormone.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody. View Larger

Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody. Recombinant Rat Growth Hormone R/GHR Fc Chimera (Catalog # 1211‑GR) inhibits Recombinant Human Growth Hormone (Catalog # 1067‑GH) induced proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human Growth Hormone (0.2 ng/mL) activity elicited by Recombinant Rat Growth Hormone R/GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Rat Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1211). The ND50 is typically 0.075‑0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Rat GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with a 18 aa signal peptide, a 247 aa extracellular domain, a 24 aa transmembrane domain and a 349 aa cytoplasmic domain. An alternatively spliced 297 aa isoform of rat GHR also exists. This 279 aa variant corresponds to the serum GH-binding protein and is identical in sequence to the extracellular domain of the transmembrane protein up to Glu262 (5). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (6). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (5, 7).

References
  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.
  6. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  7. Frick, G.P. et al. (1998) Endocrinology 139:2824.
Long Name
Growth Hormone Receptor
Entrez Gene IDs
2690 (Human); 14600 (Mouse); 25235 (Rat)
Alternate Names
GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor

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Immunohistochemistry Reagents

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Secondary Antibodies

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