TGF-beta 1 Antibody

(46 citations)   
  • Specificity
    Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than
    2% cross-reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 9016
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TGF-beta 1 (Catalog # 240-B)
    under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
    • Human TGF-beta 1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    TGF‑ beta 1 Antibody (Catalog # MAB240)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    TGF‑ beta 1 Biotinylated Antibody (Catalog # BAF240)
  • ELISA Standard
     
    Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1 Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240). The ND50 is typically 0.3-1.0 µg/mL.
Intracellular Staining by Flow Cytometry
Detection of TGF‑ beta 1 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Immunohistochemistry
TGF-beta 1 in Human Prostate Cancer Tissue. TGF-beta 1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-
TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in the prostate gland. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

  • Long Name:
    Transforming Growth Factor beta 1
  • Entrez Gene IDs:
    7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
  • Alternate Names:
    CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
Sample Type
  1. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015;10(6):e0130395.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015;195(5):2141-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
    Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
    Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-ss
    Authors: Ogawa H, Ledford J, Mukherjee S, Aono Y, Nishioka Y, Lee J, Izumi K, Hollingsworth J
    Respir Res, 2014;15(1):143.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Regulation of renal fibrosis by Smad3 Thr388 phosphorylation.
    Authors: Qu X, Li X, Zheng Y, Ren Y, Puelles V, Caruana G, Nikolic-Paterson D, Li J
    Am J Pathol, 2014;184(4):944-52.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  6. Mesenchymal stem cells cancel azoxymethane-induced tumor initiation.
    Authors: Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y
    Stem Cells, 2014;32(4):913-25.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  7. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
    Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
    J Immunol, 2014;192(2):804-16.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  8. TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement.
    Authors: Bialas A, Stevens B
    Nat Neurosci, 2013;16(12):1773-82.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Immunodepletion
  9. Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction.
    Authors: Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z
    Eur J Immunol, 2013;43(9):2461-72.
    Species: Mouse
    Sample Type: exosomes
    Application: Neut
  10. Reduction of methylglyoxal-induced glycation by pyridoxamine improves adipose tissue microvascular lesions.
    Authors: Rodrigues T, Matafome P, Santos-Silva D, Sena C, Seica R
    J Diabetes Res, 2013;2013(0):690650.
    Species: Rat
    Sample Type: tissue
    Application: IHC
  11. Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis.
    Authors: Zhu Y, Colak T, Shenoy M, Liu L, Mehta K, Pai R, Zou B, Xie X, Pasricha P
    Mol Pain, 2012;8(0):65.
    Species: Rat
    Sample Type: In Vivo
    Application: Neut
  12. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2012;185(6):3529-35.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  13. HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages.
    Authors: Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay MJ
    PLoS ONE, 2012;7(3):e32761.
    Species: Human
    Sample Type: recombinant TGF-beta1
    Application: Neut
  14. Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
    Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
    J. Biol. Chem., 2011;286(20):17435-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  15. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011;121(11):4548-66.
    Species: Human
    Sample Type: recombinant TGF-b1
    Application: WB
  16. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
    Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
    Mol. Biol. Cell, 2011;22(10):1686-98.
    Species: Canine
    Sample Type: Whole Cells
    Application: Neut
  17. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011;54(10):2713-23.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  18. AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection.
    Authors: Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J
    J. Immunol., 2010;184(2):984-92.
    Species: Primate - Macaca mulatta (Rhesus monkey)
    Sample Type: Whole Tissue
    Application: IHC Frozen
  19. Activation of Toll-like receptor 3 augments myofibroblast differentiation.
    Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M
    Am. J. Respir. Cell Mol. Biol., 2009;40(6):654-62.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  20. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
    Authors: Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L
    J. Exp. Med., 2009;206(4):819-31.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  21. Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection.
    Authors: Bhowmick S, Mazumdar T, Ali N
    Infect. Immun., 2009;77(4):1514-23.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  23. Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction.
    Authors: Meltendorf C, Burbach GJ, Ohrloff C, Ghebremedhin E, Deller T
    Invest. Ophthalmol. Vis. Sci., 2009;50(8):3688-95.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC Frozen
  24. Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells.
    Authors: Pot C, Jin H, Awasthi A
    J. Immunol., 2009;183(2):797-801.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  25. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008;26(4):1047-55.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  26. Transforming growth factor-beta stimulates intestinal epithelial focal adhesion kinase synthesis via smad- and p38-dependent mechanisms.
    Authors: Walsh MF, Ampasala DR, Hatfield J, Vander Heide R, Suer S, Rishi AK, Basson MD
    Am. J. Pathol., 2008;173(2):385-99.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  27. Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection.
    Authors: Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z
    J. Immunol., 2008;180(1):113-21.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  28. IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells.
    Authors: Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA
    J. Immunol., 2007;178(4):2018-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  29. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  30. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007;8(9):942-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  31. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
    Authors: Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB
    J. Immunol., 2007;178(9):5552-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  32. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2007;109(5):2234-42.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Array Development
  33. Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
    Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
    Immunol. Lett., 2006;105(2):159-66.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  34. Increased frequency of CD4+ cells expressing CD161 in cancer patients.
    Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA
    Clin. Cancer Res., 2006;12(23):6901-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  35. Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy.
    Authors: Li J, Campanale NV, Liang RJ, Deane JA, Bertram JF, Ricardo SD
    Am. J. Pathol., 2006;169(5):1527-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  36. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  37. Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma.
    Authors: Yang XH, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, Sato Y, Abo T, Horwitz DA, Aoyagi Y
    J. Hepatol., 2006;45(2):254-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  38. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  39. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  40. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  41. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: Human
    Sample Type: Serum
    Application: Affinity Chromatography
  42. Induction of a hypertrophic growth status of coronary smooth muscle cells is associated with an overexpression of TGF-beta.
    Authors: Schmidt A, Gopfert C, Vlodavsky I, Volker W, Buddecke E
    Eur. J. Cell Biol., 2002;81(3):138-44.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut
  43. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  44. Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
    Authors: Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD
    J. Biol. Chem., 2002;277(47):45547-57.
    Species: Human
    Sample Type: Cell Lysates
    Application: Neut
  45. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  46. Isoform specificity of commercially-available anti-TGF-beta antibodies.
    Authors: Mozes MM, Hodics T, Kopp JB
    J. Immunol. Methods, 1999;225(1):87-93.
    Species: Human
    Sample Type: recombinant TGFb1
    Application: WB
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