TGF-beta 1 Antibody (9016EC)

Name: TGF-beta 1 Antibody (9016EC)
Brand: R&D Systems
SKU: MAB2402
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB2402

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Citations (3)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse, Feline

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 9016EC

View all TGF-beta 1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1

Specificity

Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for TGF-beta 1 Antibody (9016EC)

Mouse TGF-beta 1 ELISA Standard Curve

Recombinant Human TGF‑ beta RII Fc Chimera (Catalog # 341-BR) was serially diluted and captured by Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB2402) coated on a Clear Polystyrene Microplate (Catalog # DY990). Chicken Anti-TGF‑ beta 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Detection of TGF-beta 1 by Western Blot

Mitigation of liver fibrosis by 6-shogaol in MCD-diet-fed mice. (A) Masson’s trichrome staining of liver tissues. Scale bar = 100 μm. (B) Percentage of areas exhibiting Masson’s trichrome staining. (C) Western blots displaying fibronectin, vimentin, TGF-beta 1, and alpha -SMA in liver tissues. (D) Quantitative analysis of Western blot results. (E) IHC staining of liver tissues for alpha -SMA. Scale bar = 100 μm. (F) Percentage of areas displaying alpha -SMA staining. n = 8 per group. ** p < 0.01 and *** p < 0.001 vs. the Con group. #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. the MCD group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38257332), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF-beta 1 Antibody (9016EC)

Human TGF-beta 1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: TGF‑ beta 1 Biotinylated Antibody (Catalog # BAF240)
Standard: Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta 1

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.