Viral EBV IL-10 Antibody (134216)

Name: Viral EBV IL-10 Antibody (134216)
Brand: R&D Systems
SKU: MAB915
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # MAB915

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Virus

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 134216

View all IL-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant viral EBV IL‑10
Gln26-Arg170
Accession # P03180

Specificity

Detects viral EBV IL‑10 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant mouse IL-10, recombinant porcine IL-10, and recombinant rat IL-10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Viral EBV IL-10 Antibody (134216)

Cell Proliferation Induced by IL‑10 and Neutralization by Viral IL‑10 Antibody.

Recombinant Viral EBV IL-10 (Catalog # 915-VL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL-10 Monoclonal Antibody (Catalog # MAB915). The ND₅₀ is typically 0.1-0.4 µg/mL.

Applications for Viral EBV IL-10 Antibody (134216)

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Viral EBV IL‑10 (Catalog # 915-VL)

Neutralization

Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson‑Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.1‑0.4 µg/mL in the presence of 100 ng/mL Recombinant Viral EBV IL‑10.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-10

Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)