Detects viral EBV IL‑10 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant mouse IL-10, recombinant porcine IL-10, and recombinant rat IL-10 is observed.
Monoclonal Mouse IgG2B Clone # 134216
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant viral EBV IL‑10 Gln26-Arg170 Accession # P03180
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson‑Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 100 ng/mL Recombinant Viral EBV IL‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑10 and Neutralization by Viral IL‑10 Antibody. Recombinant Viral EBV IL‑10 (Catalog # 915-VL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL‑10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL‑10 Monoclonal Antibody (Catalog # MAB915). The ND50 is typically 0.1‑0.4 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.