Key Product Details

Species Reactivity

Viral

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 994717
View all Yellow Fever Virus NS1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293­-derived Yellow Fever Virus NS1
Gln780-His1134
Accession # P03314

Specificity

Detects Yellow Fever Virus NS1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral Yellow Fever Virus NS1 Antibody

Viral Yellow Fever Virus NS1 ELISA Standard Curve.

Recombinant Viral Yellow Fever Virus NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB97761) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB97762) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Viral Yellow Fever Virus NS1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB97762).This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Yellow Fever Virus NS1

Yellow fever virus (YFV) non-structural protein 1 (NS1) is produced by yellow fever virus (YFV), a mosquito-borne flavivirus that is responsible for numerous epidemics of yellow fever disease with high fatality rates (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane‑associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral YFV NS1 contains 352 amino acids (afa) and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References

  1. Beasley, D.W. et al. (2015) Antiviral. Res. 115:48.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Alternate Names

YFV NS1, YFV-NS1, YFV_NS1

Entrez Gene IDs

1502173 (Viral)

Gene Symbol

POLY

UniProt

P03314

Additional Yellow Fever Virus NS1 Products

Product Documents for Viral Yellow Fever Virus NS1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral Yellow Fever Virus NS1 Antibody

For research use only

Related Research Areas

Infectious Diseases

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