Zebrafish VEGF Antibody

Catalog # Availability Size / Price Qty
MAB1247
MAB1247-SP
Cell Proliferation Induced by VEGF165 and Neutralization by Zebrafish VEGF Antibody.
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Product Details
Citations (2)
FAQs
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Zebrafish VEGF Antibody Summary

Species Reactivity
Zebrafish
Specificity
Detects zebrafish VEGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) VEGF121, 165, 206, rhVEGF-B186, rhVEGF-C, rhVEGF‑D, recombinant mouse (rm) VEGF115, rmVEGF165,  rmVEGF-B186, rmVEGF‑D, or recombinant rat VEGF164 is observed.
Source
Monoclonal Mouse IgG2B Clone # 211615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant zebrafish VEGF165
Ala24-Arg188
Accession # O73682.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Zebrafish VEGF165 (Catalog # 1247-ZV)
Neutralization
Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.2-0.5 µg/mL in the presence of 100 ng/mL Recombinant Zebraship VEGF165.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by VEGF165 and Neutralization by Zebrafish VEGF Antibody. View Larger

Cell Proliferation Induced by VEGF165 and Neutralization by Zebrafish VEGF Antibody.

Recombinant Zebrafish VEGF165 (Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF165 (100 ng/mL) is neutral­lized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND50 is typically 0.2‑0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF165 shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF165, respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish (FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as upregulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1‑7).

References
  1. Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14.
  2. Laing, D. and R. Ge (1998) GenBank Accession # AAC14713.
  3. Laio, W. et al. (1997) Development 124:381.
  4. Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470.
  5. Thurston, G. (2002) J. Anat. 200:575.
  6. Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
  7. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Product Datasheets

Citations for Zebrafish VEGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis.
    Authors: Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA
    J. Biol. Chem., 2008;283(23):16084-92.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology.
    Authors: Vigneswaran N, Sacks P, Gilcrease M, Zacharias W
    J. Oral Pathol. Med., 2005;34(2):77-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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