Feline IL-5 Antibody

Catalog # Availability Size / Price Qty
AF1795
AF1795-SP
Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody.
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Feline IL-5 Antibody Summary

Species Reactivity
Feline
Specificity
Detects feline IL-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant bovine IL-5 and recombinant equine IL-5 is observed, approximately 50% cross-reactivity with recombinant porcine IL-5 and recombinant canine IL-5 is observed, approximately 10% cross-reactivity with recombininat human IL-5 is observed, and less than 1% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant rhesus monkey IL-5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant feline IL-5
Ile20-Ser134
Accession # O77515
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Feline IL‑5 (Catalog # 1795-FL)
Immunocytochemistry
5-15 µg/mL
Immersion fixed feline peripheral blood mononuclear cells
Neutralization
Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 30 ng/mL Recombinant Feline IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody. View Larger

Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody. Recombinant Feline IL‑5 (Catalog # 1795-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1795). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin 5 (IL-5) is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 is also a growth and differentiation factor for B cells (1‑3). Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. As in human IL-5, the cDNA for cat IL-5 encodes a precursor protein with signal peptide that is cleaved to generate the secreted mature protein containing 115 amino acid (aa) residues. Feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. IL-5 exerts its activity on target cells by binding to specific cell surface receptor complexes. The functional high-affinity receptor complex for IL-5 is composed of a ligand-binding alpha subunit that is specific for IL-5, and a non ligand-binding common beta subunit that is required for signal transduction. The common beta subunit is shared with the high-affinity receptor complexes for IL-3 and GM-CSF. In human, IL-5 R alpha subunit is primarily expressed on eosinophils and basophils. During eosinophil development, IL-5 up‑regulates the expression of IL-5 R alpha. In contrast, in mature eosinophils, the expression of IL-5 R alpha mRNA is down-regulated by IL-5, as well as by IL-3 and GM-CSF. Furthermore, IL-5 also down-modulates cell surface IL-5 R alpha via a proteinase-mediated process that releases the soluble IL-5 R alpha extracellular domain (4‑6).

References
  1. Karlen, S. et al. (1998) Int. Rev. Immunol. 16:227.
  2. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
  3. Takatsu, K. (1998) Cytokine Growth Factor Rev. 9:25.
  4. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  5. Hellman, C. et al. (2003) Clin. Exp. Immunol. 131:75.
  6. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.
Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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