Human 20S Proteasome Antibody (863437)

R&D Systems | Catalog # MAB9554

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 863437
View all 20S Proteasome Primary Antibodies »

Product Specifications

Immunogen

Human 20S Proteasome purified from human erthrocytes

Specificity

Detects human 20S Proteasome in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human 20S Proteasome Antibody (863437)

Detection of Human 20S Proteasome antibody by Western Blot.

Detection of Human 20S Proteasome by Western Blot.

Western blot shows Human 20S Proteasome (Catalog # E-360), Human 20S Immunoproteasome (Catalog # E-370), and Recombinant Human PSMA1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human 20S Proteasome Monoclonal Antibody (Catalog # MAB9554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 20S Proteasome at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
20S Proteasome antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

20S Proteasome in A549 Human Cell Line.

20S Proteasome was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human 20S Proteasome Monoclonal Antibody (Catalog # MAB9554) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human 20S Proteasome Antibody (863437)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Western Blot

2 µg/mL
Sample: Human 20S Proteasome (Catalog # E-360), Human 20S Immunoproteasome (Catalog # E-370), and Recombinant Human PSMA1

Reviewed Applications

Read 1 review rated 1 using MAB9554 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 20S Proteasome

The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings. However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1. The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes. 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.

Additional 20S Proteasome Products

Product Documents for Human 20S Proteasome Antibody (863437)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 20S Proteasome Antibody (863437)

For research use only

Citations for Human 20S Proteasome Antibody (863437)

Customer Reviews for Human 20S Proteasome Antibody (863437) (1)

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  • Human 20S Proteasome Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 04/29/2021
    Figure shows signal detected (or lack thereof) from an ELISA using a serial dilution of brain lysates (high to low concentrations, top to bottom) and different concentrations of the antibody (left to right)
    Human 20S Proteasome Antibody (863437) MAB9554

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