Human A20/TNFAIP3 Antibody

Detection of Human A20/TNFAIP3 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI-H460 human large cell lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for A20/TNFAIP3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of A20/TNFAIP3 in Human Colon.

A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells in mucosal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human A20/TNFAIP3 by Simple Western^TM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cells, HepG2 human hepatocellular carcinoma cells, and NCI‑H460 human large cell lung carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for A20/TNFAIP3 at approximately 101 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.