Detection of Human A20/TNFAIP3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI‑H460 human large cell lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for A20/TNFAIP3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
A20 (TNF alpha -induced protein 3) is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.
Entrez Gene IDs:
7128 (Human); 21929 (Mouse)
A20TNFA1P2; EC 220.127.116.11; EC 6.3.2.-; MGC104522; MGC138687; OTU domain-containing protein 7C; OTUD7C; OTUD7CMGC138688; Putative DNA-binding protein A20; TNF alpha-induced protein 3; TNFA1P2; TNFAIP3; tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20
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