Human ABI1 Antibody

Catalog # Availability Size / Price Qty
MAB10249-100
MAB10249-SP
ABI1  in A549 Human Cell Line.
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Human ABI1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ABI1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1013203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ABI1
Val429-Asp508
Accession # Q8IZP0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry ABI1  in A549 Human Cell Line. View Larger

ABI1 in A549 Human Cell Line. ABI1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABI1 Monoclonal Antibody (Catalog # MAB10249) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ABI1  in Human Brain Tissue. View Larger

ABI1 in Human Brain Tissue. ABI1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human ABI1 Monoclonal Antibody (Catalog # MAB10249) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABI1

Abelson Interactor 1 (ABI1) is an adaptor protein involved in cytoskeletal reorganization and EGFR signaling. Overexpression of ABI1 inhibits EGF-induced activation of Erks which negatively regulate cell growth and transformation. In the brain, ABI1 seems to regulate dendritic outgrowth and determines the shape and number of synaptic contacts of developing neurons.

References
  1. UniProt: The Universal Protein Knowledgebase. (2017)  Nucleic Acids Res. 45: D158-D169.
  2. Fan. P.-D. and S.P. Goff (2000) Mol. Cell. Biol. 20:7591.
  3. Xiong X. et al. (2008) Biochim Biophy. Acta 1783:737.
Long Name
Abl Interactor 1
Entrez Gene IDs
10006 (Human)
Alternate Names
Abelson interactor 1; ABI1; ABI-1; abl interactor 1; Abl-binding protein 4; ABLBP4; abl-interactor 1; Abl-interactor protein 1 long; E3B1; eps8 binding protein; Eps8 SH3 domain-binding protein; Eps8-binding protein; interactor protein AblBP4; nap1 binding protein; Nap1-binding protein; NAP1BP; spectrin SH3 domain binding protein 1; Spectrin SH3 domain-binding protein 1; SSH3BP; SSH3BP1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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