Human/Hamster ACE‑2 Antibody

R&D Systems | Catalog # MAB933

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Hamster

Cited:

Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 171606
View all ACE-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740 (predicted)
Accession # Q9BYF1

Specificity

Detects human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ACE-1 or rhNeprilysin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Hamster ACE‑2 Antibody

Detection of Human ACE-2 antibody by Western Blot.

Detection of Human ACE‑2 by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ACE-2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

ACE‑2 in Human Kidney.

ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ACE‑2 antibody in Hamster Lung by Immunohistochemistry (IHC-P).

ACE‑2 in Hamster Lung.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human ACE-2 by Immunohistochemistry

Detection of Human ACE-2 by Immunohistochemistry

In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative image of a pancreatic section showing two adjacent lobules (blue and red dotted lines) with different staining for ACE2 in endothelial cells/pericytes. A specific segmentation of the two lobules with high (blue) (zoom-in, panel-b) and low or null expression of ACE2 (red) (zoom-in, panel-c) is shown, suggesting lobularity of ACE2 expression in exocrine endothelial cells/pericytes of human pancreas. Scale bar in panel-a: 100 µm. Scale bar in panels-b and -c: 30 µm. (B) Double immunofluorescence staining of ACE2 (green) and CD31 (red) in FFPE pancreas sections from Body01A of Case #110118 (panels-a to -d) and of Body01B of Case #141117 (panels-e to -i). Digital zoom-in overlay images are shown in panels-d, -h and -i. Scale bar in panels-d and -g: 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33281748), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ACE-2 by Immunohistochemistry

Detection of Human ACE-2 by Immunohistochemistry

ACE2 staining pattern in human pancreas. Immunohistochemistry for ACE2 in human pancreatic tissue sections (case #110118) using R&D MAB933 antibody. ACE2 is markedly expressed in microvasculature associated cells (A, B) in some rare ductal cells (C, D) and in a subset of endocrine cells within pancreatic islets (E, F). Scale bars in (A, C, E) 150 µm. Scale bars in (B, D, F) 70 µm. Zoom-in images are reported in (B, D, F). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33281748), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ACE-2 by Immunohistochemistry

Detection of Human ACE-2 by Immunohistochemistry

In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative image of a pancreatic section showing two adjacent lobules (blue and red dotted lines) with different staining for ACE2 in endothelial cells/pericytes. A specific segmentation of the two lobules with high (blue) (zoom-in, panel-b) and low or null expression of ACE2 (red) (zoom-in, panel-c) is shown, suggesting lobularity of ACE2 expression in exocrine endothelial cells/pericytes of human pancreas. Scale bar in panel-a: 100 µm. Scale bar in panels-b and -c: 30 µm. (B) Double immunofluorescence staining of ACE2 (green) and CD31 (red) in FFPE pancreas sections from Body01A of Case #110118 (panels-a to -d) and of Body01B of Case #141117 (panels-e to -i). Digital zoom-in overlay images are shown in panels-d, -h and -i. Scale bar in panels-d and -g: 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33281748), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ACE-2 by Immunocytochemistry/Immunofluorescence

Detection of Human ACE-2 by Immunocytochemistry/Immunofluorescence

In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative image of a pancreatic section showing two adjacent lobules (blue and red dotted lines) with different staining for ACE2 in endothelial cells/pericytes. A specific segmentation of the two lobules with high (blue) (zoom-in, panel-b) and low or null expression of ACE2 (red) (zoom-in, panel-c) is shown, suggesting lobularity of ACE2 expression in exocrine endothelial cells/pericytes of human pancreas. Scale bar in panel-a: 100 µm. Scale bar in panels-b and -c: 30 µm. (B) Double immunofluorescence staining of ACE2 (green) and CD31 (red) in FFPE pancreas sections from Body01A of Case #110118 (panels-a to -d) and of Body01B of Case #141117 (panels-e to -i). Digital zoom-in overlay images are shown in panels-d, -h and -i. Scale bar in panels-d and -g: 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33281748), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Chlorocebus sabaeus ACE-2 by Immunocytochemistry/ Immunofluorescence

Detection of Chlorocebus sabaeus ACE-2 by Immunocytochemistry/ Immunofluorescence

Modulation of cell surface-expressed ATR1 and ACE2 molecules in Vero E6 cells infected with SARS-CoV-2 (24 h.p.i) and treated with Azilsartan (15 µM) for 72 hours by immunofluorescence microscopy. (A) Schematic flow of the analysis: Vero E6 cells were treated with various ARBs (the MTT assay previously defined non-cytotoxic concentrations: Azilosartan 15µM; Eprosartan 30µM; Irbesartan 60µM; Losartan 7µM; Olmesartan 15µM; Telmisartan 7µM; Valsartan 7µM) for 72 hours and were subsequently infected for analysis of ACE2 and ATR1 on treated and infected cells, 24 hours post-infection (h.p.i.). (B) The panel presents SARS-CoV-2 infected cells after incubation with Azilsartan (15 µM) and evaluation of fluorescence corresponding to the ATR1, ACE2, viral spike protein, and the nucleus of the cells. The merge of the images is displayed at the right of the panel. Images were acquired using a confocal microscope (Zeiss LSM 800) with a 63X/1.4 oil objective. (C) Quantitative representation of Mean Fluorescence corresponding to ATR1 and ACE2 molecules expression on VERO E6 cells treated or not treated with Azilsartan and SARS-CoV-2 in the cells. ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34178717), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Hamster ACE‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and hamster lung.

Western Blot

2 µg/mL
Sample: NS0 mouse myeloma cell line and human kidney tissue

Reviewed Applications

Read 4 reviews rated 4.5 using MAB933 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

References

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Gene Symbol

ACE2

UniProt

Q9BYF1

Additional ACE-2 Products

Product Documents for Human/Hamster ACE‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Hamster ACE‑2 Antibody

For research use only

Citations for Human/Hamster ACE‑2 Antibody

Customer Reviews for Human/Hamster ACE‑2 Antibody (4)

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Showing  1 - 4 of 4 reviews Showing All
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  • Human/Hamster ACE-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 11/26/2021
    Human/Hamster ACE‑2 Antibody MAB933
  • Human/Hamster ACE-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 10/19/2021
    Human/Hamster ACE‑2 Antibody MAB933
  • Human/Hamster ACE-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Small intestine
    Species: Human
    Verified Customer | Posted 08/04/2021
    Human/Hamster ACE‑2 Antibody MAB933
  • Human/Hamster ACE-2 Antibody
    Name: deng guo
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mouse IMCD3 cells
    Species: Human ACE2
    Verified Customer | Posted 09/28/2020
    IMCD3 cells were transiently transfected with human ACE2. 48 hrs post transfection, the cells were fixed, then stain with the ACE2 antibody (1:250 dilution).
    Human/Hamster ACE‑2 Antibody MAB933

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Protocols

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FAQs for Human/Hamster ACE‑2 Antibody

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  • Q: Does Human/Hamster ACE-2 Antibody, Catalog# MAB933, detect mouse ACE2?

    A: We have not evaluated cross-reactivity of this antibody to mouse ACE-2. Human ACE-2 shares 83% amino acid sequence identity with mouse ACE-2. We have one citation on our webpage where researcher reports using MAB933 to detect mouse ACE-2. 

  • Q: Is Catalog # MAB933 kappa or lambda light chain?

    A: MAB933 is kappa light chain.

  • Q: Does Human/Hamster ACE-2 Antibody, Catalog# MAB933, detect mouse ACE2?

    A: We have not evaluated cross-reactivity of this antibody to mouse ACE-2. Human ACE-2 shares 83% amino acid sequence identity with mouse ACE-2. We have one citation on our webpage where researcher reports using MAB933 to detect mouse ACE-2. 

  • Q: Is Catalog # MAB933 kappa or lambda light chain?

    A: MAB933 is kappa light chain.

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