Human ACE-2 Antibody

(17 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ACE-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ACE-2
    Gln18-Ser740
    Accession # Q9BYF1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
ACE‑2 in Human Kidney. ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
ACE‑2 in Human Kidney. ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human ACE‑2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

  • References:
    1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
    2. Crackower,  M.A. et al. (2002) Nature 417:822.
    3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  • Long Name:
    Angiotensin I Converting Enzyme 2
  • Entrez Gene IDs:
    59272 (Human); 70008 (Mouse); 302668 (Rat)
  • Alternate Names:
    ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HTCC: Broad Range Inhibitor of Coronavirus Entry
    Authors: Aleksandra Milewska
    PLoS ONE, 2016;11(6):e0156552.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: IHC Not Specified
  2. Surface vimentin is critical for the cell entry of SARS-CoV.
    Authors: Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M
    J Biomed Sci, 2016;23(1):14.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Whole Cells
    Application: Neut
  3. Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells.
    Authors: Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K
    PLoS ONE, 2014;9(1):e85958.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
    Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
    J Virol, 2014;88(2):1293-307.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
    Authors: Ge, Xing-Yi, Li, Jia-Lu, Yang, Xing-Lou, Chmura, Aleksei, Zhu, Guangjia, Epstein, Jonathan, Mazet, Jonna K, Hu, Ben, Zhang, Wei, Peng, Cheng, Zhang, Yu-Ji, Luo, Chu-Ming, Tan, Bing, Wang, Ning, Zhu, Yan, Crameri, Gary, Zhang, Shu-Yi, Wang, Lin-Fa, Daszak, Peter, Shi, Zheng-Li
    Nature, 2013;503(7477):535-8.
    Species: Bat
    Sample Type: Whole Cells
    Application: IF
  6. In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
    Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C
    Virol J, 2013;10(0):359.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  7. Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts.
    Authors: Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ
    PLoS ONE, 2012;7(4):e35876.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron.
    Authors: Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F
    J. Biol. Chem., 2010;285(53):41935-46.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system.
    Authors: Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S
    J. Am. Coll. Cardiol., 2008;52(9):750-4.
    Species: Human
    Sample Type: Plasma
    Application: Neut
  10. Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.
    Authors: Lu Y, Liu DX, Tam JP
    Biochem. Biophys. Res. Commun., 2008;369(2):344-9.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Whole Cells
    Application: Flow
  11. Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2.
    Authors: Glende J, Schwegmann-Wessels C, Al-Falah M, Pfefferle S, Qu X, Deng H, Drosten C, Naim HY, Herrler G
    Virology, 2008;381(2):215-21.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Cell Lysates
    Application: WB
  12. Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin.
    Authors: Ren W, Qu X, Li W, Han Z, Yu M, Zhou P, Zhang SY, Wang LF, Deng H, Shi Z
    J. Virol., 2007;82(4):1899-907.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted.
    Authors: Inoue Y, Tanaka N, Tanaka Y, Inoue S, Morita K, Zhuang M, Hattori T, Sugamura K
    J. Virol., 2007;81(16):8722-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Cell Lysates
    Application: WB
  14. Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice.
    Authors: Rockx B, Sheahan T, Donaldson E, Harkema J, Sims A, Heise M, Pickles R, Cameron M, Kelvin D, Baric R
    J. Virol., 2007;81(14):7410-23.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Whole Cells
    Application: Neut
  15. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells.
    Authors: de Lang A, Osterhaus AD, Haagmans BL
    Virology, 2006;353(2):474-81.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Whole Cells
    Application: ICC
  16. Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells.
    Authors: Tseng CT, Tseng J, Perrone L, Worthy M, Popov V, Peters CJ
    J. Virol., 2005;79(15):9470-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  17. Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
    Authors: Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S
    Biochem. Biophys. Res. Commun., 2004;319(4):1216-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 17 Citations
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Western Blot Human ACE-2 Antibody AF933
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 WB Human Anonymous 08/12/2016
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Western Blot Human ACE-2 Antibody AF933
Western Blot: Human ACE-2 Antibody [AF933]

Summary

ApplicationWestern Blot
Sample TestedBlood mononuclear cells (PBMCs),Human CD34+ Cells
SpeciesHuman

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