alpha -Synuclein is member of a family of small soluble proteins that include also beta -, and gamma -Synuclein. It is predominantly expressed in neurons of the central nervous system in the presynaptic region of nerve terminals, where it cycles between a free, partially unfolded and a helical, membrane-bound form. alpha -Synuclein can self-aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms of alpha -Synuclein are major components of Lewy bodies and Lewy neurites and have been linked to the pathogenesis of Parkinson’s Disease, Parkinson’s Disease Dementia, and dementia with Lewy bodies. alpha -Synuclein aggregates can be also found associated with amyloid plaques in Alzheirmer’s Disease.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1060506
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Product Specifications
Immunogen
E. coli-derived human alpha-Synuclein
Met1-Ala140
Accession # P37840
Met1-Ala140
Accession # P37840
Specificity
Detects human alpha -Synuclein in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human alpha -Synuclein Antibody
Detection of alpha -Synuclein in SK‑Mel‑28 cells (positive) and HL‑60 cells (negative).
alpha -Synuclein was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and absent in HL‑60 human acute promyelocytic leukemia cells (negative) using Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB11376) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of alpha -Synuclein in Human Brain Cerebellum.
alpha -Synuclein was detected in immersion fixed paraffin-embedded sections of Human Brain Cerebellum using Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB11376) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to medulla. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human alpha -Synuclein Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and HL‑60 human acute promyelocytic leukemia cells (negative)
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and HL‑60 human acute promyelocytic leukemia cells (negative)
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Brain Cerebellum
Sample: Immersion fixed paraffin-embedded sections of Human Brain Cerebellum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: alpha-Synuclein
References
-
Breydo L. et al. (2012) Biochim. Biophys. Acta. 1822:261.
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Chen R.H. et al. (2013) J. Biol. Chem. 288:7438.
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Li X. et al. (2008) Acta Biochim. Biophys. Sin (Shanghai) 40:406.
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Surguchov A. (2008) Int. Rev. Cell Mol. Biol. 270:225.
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Xia Q. et al. (2008) Front. Biosci. 13:3850.
Alternate Names
NACP, PARK1, PARK4, SNCA, Synuclein-alpha
Gene Symbol
SNCA
UniProt
Additional alpha-Synuclein Products
Product Documents for Human alpha -Synuclein Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human alpha -Synuclein Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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