Human Angiopoietin-1 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2, recombinant mouse Angiopoietin-like 3, rhAngiopoietin-4, or rhAngiopoietin-like 7 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 171718
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
    Ser20-Phe498
    Accession # Q15389
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Angiopoietin‑1 in Human Prostate Cancer Tissue. Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa) precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% aa sequence identity with mouse Ang‑1 and human Ang‑2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1-induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

  • References:
    1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
    2. Davis, S. et al. (1996) Cell 87:1161.
    3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
    4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
    5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
  • Entrez Gene IDs:
    284 (Human); 11600 (Mouse)
  • Alternate Names:
    AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells
    Authors: Wilson Chan
    PLoS ONE, 2016;11(6):e0156994.
    Species: Human
    Sample Type: Protein
    Application: ELISA Development
  2. Early systemic microvascular damage in pigs with atherogenic diabetes mellitus coincides with renal angiopoietin dysbalance.
    Authors: Khairoun M, van den Heuvel M, van den Berg B, Sorop O, de Boer R, van Ditzhuijzen N, Bajema I, Baelde H, Zandbergen M, Duncker D, Rabelink T, Reinders M, van der Giessen W, Rotmans J
    PLoS ONE, 2015;10(4):e0121555.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. High bone marrow angiopoietin-1 expression is an independent poor prognostic factor for survival in patients with myelodysplastic syndromes.
    Authors: Cheng CL, Hou HA, Jhuang JY, Lin CW, Chen CY, Tang JL, Chou WC, Tseng MH, Yao M, Huang SY, Ko BS, Hsu SC, Wu SJ, Tsay W, Chen YC, Tien HF
    Br. J. Cancer, 2011;105(7):975-82.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. Regulation of angiopoietin expression by bacterial lipopolysaccharide.
    Authors: Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L955-63.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
    Authors: Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
    FASEB J., 2008;22(8):3010-23.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: IP
  6. Angiopoietin 1 directly induces destruction of the rheumatoid joint by cooperative, but independent, signaling via ERK/MAPK and phosphatidylinositol 3-kinase/Akt.
    Authors: Hashiramoto A, Sakai C, Yoshida K, Tsumiyama K, Miura Y, Shiozawa K, Nose M, Komai K, Shiozawa S
    Arthritis Rheum., 2007;56(7):2170-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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