Detects human Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2, recombinant mouse Angiopoietin-like 3, rhAngiopoietin-4, or rhAngiopoietin-like 7 is observed.
Monoclonal Mouse IgG2B Clone # 171718
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1 Ser20-Phe498 Accession # Q15389
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Angiopoietin‑1 in Human Prostate Cancer Tissue. Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted
ligands which bind with similar affinity to Tie-2, a receptor tyrosine
kinase with immunoglobulin and epidermal growth factor homology domains
expressed primarily on endothelial cells and early hematopoietic cells.
Tie-2 and angiopoietins have been shown to play critical roles in
embryogenic angiogenesis and in maintaining the integrity of the adult
vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa)
precursor protein that contains a coiled-coiled domain near the
amino-terminus and a fibrinogen-like domain at the C-terminus. Human
Ang-1 shares approximately 97% and 60% aa sequence identity with mouse
Ang‑1 and human Ang‑2, respectively (1, 2). Ang-1 activates Tie-2
signaling on endothelial cells to promote chemotaxis, cell survival,
cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has
alternatively been reported to be an antagonist for Ang-1-induced Tie-2
signaling as well as an agonist for Tie-2 signaling, depending on the
cell context (5).
Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
Davis, S. et al. (1996) Cell 87:1161.
Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.