Human Angiopoietin-2 Antibody

  (2 citations)
(2 Reviews)
    
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Product Details
Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Angiopoietin-2 in ELISAs. In ELISAs, no cross-reactivity or interference with recombinant human (rh) Ang‑1, rhAng-4, rhAng-X, recombinant mouse (rm) Ang-3, rmANGPTL-3, rhTie-1, rhTie-2, and rmTie-2 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 85816
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-2
    Asp68-Phe496
    Accession # O15123
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
    • Human Angiopoietin-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Angiopoietin‑2 Antibody (Catalog # MAB098 )
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human Angiopoietin‑2 Biotinylated Antibody (Catalog # BAM0981 )
  • ELISA Standard
     
    Recombinant Human Angiopoietin-2 Protein (Catalog # 623-AN )
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled-coil domain (aa 166 - 248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275 - 495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65 - 66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250 - 300 kDa tetramers and pentamers (3 - 6). Alternate splicing generates a short isoform that lacks 52 amino acids (aa) preceding the coiled-coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang-1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist (3, 8 - 11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17 - 20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).

  • References:
    1. Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165.
    2. Murdoch, C. et al. (2007) J. Immunol. 178:7405.
    3. Maisonpierre, P.C. et al. (1997) Science 27:55.
    4. Kim, I. et al. (2000) J. Biol. Chem. 275:18550.
    5. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196.
    6. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
    7. Gale, N.W. et al. (2002) Dev. Cell 3:411.
    8. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011.
    9. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159.
    10. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772.
    11. Kim, I. et al. (2001) Cardiovasc. Res. 49:872.
    12. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205.
    13. Cao, Y. et al. (2007) Cancer Res. 67:3835.
    14. Nasarre, P. et al. (2009) Cancer Res. 69:1324.
    15. Dellinger, M. et al. (2008) Dev. Biol. 319:309.
    16. Fiedler, U. et al. (2006) Nat. Med. 12:235.
    17. Koga, K. et al. (2001) Cancer Res. 61:6248.
    18. Etoh, T. et al. (2001) Cancer Res. 61:2145.
    19. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989.
    20. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680.
    21. Hu, B. et al. (2006) Cancer Res. 66:775.
    22. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.
  • Entrez Gene IDs:
    285 (Human); 11601 (Mouse)
  • Alternate Names:
    AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.
    Authors: Vart RJ, Nikitenko LL, Lagos D, Trotter MW, Cannon M, Bourboulia D, Gratrix F, Takeuchi Y, Boshoff C
    Cancer Res., 2007;67(9):4042-51.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.
    Authors: Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
    Nat. Med., 2006;12(11):1286-93.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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Isotype Controls
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Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 9
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse IgG2A Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Mouse IgG2A Antibody

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Rat Anti-Mouse IgG2A APC-conjugated Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

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Average Rating: 4 (Based on 2 reviews)

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We have 2 review tested in 1 species: Human.
We have 2 review tested in 1 application: ELISA.

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  Very Good
 ELISA Human Anonymous 12/06/2017
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Summary

Application ELISA
Sample Tested Recombinant protein
Species Human
ELISA Human Angiopoietin-2 Antibody MAB098 Array MAB098
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Very Good
 ELISA Human Anonymous 11/16/2017
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ELISA Human Angiopoietin-2 Antibody MAB098
ELISA: Human Angiopoietin-2 Antibody [MAB098].

Summary

Application ELISA
Sample Tested Serum and Plasma
Species Human

Other Experimental Details

Other Experimental Details This antibody was paired with BAM0981 to build an ELISA to measure ANG-2 in human serum samples.

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