Detects human Annexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5%
cross-reactivity with recombinant human Annexin A1, A2, A4, A6, A8, A9, A10,
A11, or A13 is observed.
Monoclonal Mouse IgG2B Clone # 693510
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Annexin A3 Ala2-Asp323 Accession # P12429
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Annexin A3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Annexin A3 in Human Prostate. Annexin A3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A3
Annexin A3 (ANXA3), also known as Annexin III and Lipocortin III (LPC3), is a 36 kDa member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing calcium-dependent phospholipid-binding sites. The C-terminal domain is comprised of four 60-70 amino acid (aa) annexin repeats. Annexin A3 is upregulated in microglia following stroke and in parenchymal hepatocytes in regenerating liver. It can be up- or down‑regulated in various carcinomas. Alternate splicing generates an additional isoform with a 30 amino acid (aa) deletion near the N-terminus. Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively.
Entrez Gene IDs:
306 (Human); 11745 (Mouse); 25291 (Rat)
35-alpha calcimedin; Annexin A3; annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase; Annexin III; annexin-3; ANXA3; Calcimedin 35-alpha ; calcimedin 35-alpha; Lipocortin III; PAP-III; Placental anticoagulant protein III; placental anticoagulant protein III, calcimedin 35-alpha
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