Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 694624
View all Annexin A4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Annexin A4
Met1-Asp319
Accession # P09525

Specificity

Detects human Annexin A4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A5, A6, A8, A9, A10, A11, or 13A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Annexin A4 Antibody

Detection of Human, Mouse, and Rat Annexin A4 antibody by Western Blot.

Detection of Human, Mouse, and Rat Annexin A4 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Annexin A4 antibody in Human Kidney by Immunohistochemistry (IHC-P).

Annexin A4 in Human Kidney.

Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and the nuclear envelope of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Annexin A4 by Simple WesternTM.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A4 at approximately 41 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human Annexin A4 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin A4

Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II, and zymogen granule membrane-associated protein of 36 kDa (ZAP36), is a member of the Annexin protein family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Human Annexin A4 shares 92% aa sequence identity with mouse and rat Annexin A4.

Alternate Names

AIV, Annexin IV, ANX4, ANXA4, Lipocortin IV, PIG28, ZAP36

Entrez Gene IDs

307 (Human); 11746 (Mouse); 79124 (Rat)

Gene Symbol

ANXA4

UniProt

P09525

Additional Annexin A4 Products

Product Documents for Human Annexin A4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Annexin A4 Antibody

For research use only

Citations for Human Annexin A4 Antibody

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Protocols

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