Detection of Human, Mouse, and Rat Annexin A4 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Annexin A4 in Human Kidney.
Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and the nuclear envelope of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A4
Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II, and zymogen granule membrane-associated protein of 36 kDa (ZAP36), is a member of the Annexin protein family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Human Annexin A4 shares 92% aa sequence identity with mouse and rat Annexin A4.
Entrez Gene IDs:
307 (Human); 11746 (Mouse); 79124 (Rat)
AIV; Annexin A4; annexin IV (placental anticoagulant protein II); Annexin IV; annexin-4; ANX4; ANX4Carbohydrate-binding protein p33/p41; ANXA4; Chromobindin-4; chromobindin-4,35-beta calcimedin; DKFZp686H02120; Endonexin I; Lipocortin IV; MGC75105; P32.5; PAP-II; PIG28; Placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; Protein II; ZAP36
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