Human BAFF R/TNFRSF13C Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BAFF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BAFF R/TNFRSF13C
    Arg2-Ala71
    Accession # Q96RJ3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog # 1162-BR)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize BAFF R/TNFRSF13C-mediated inhibition of proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 0.3 µg/mL Recombinant Human BAFF R/TNFRSF13C Fc Chimera and 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BAFF R/TNFRSF13C in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by APC-conjugated anti-goat secondary antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins
BAFF R/TNFRSF13C Inhibition of BAFF/BLys/
TNFSF13B-dependent Cell Proliferation and Neutralization by Human BAFF R/TNFRSF13C Antibody.
Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog #
1162‑BR) inhibits Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149‑BF) induced proliferation in mouse B cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) activity elicited by Recombinant Human BAFF R/TNFRSF13C Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti‑Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162). The ND50 is typically
3-15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAFF R/TNFRSF13C

B cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B cell development. While the function of BCMA is not defined, TACI has been shown to control B cell homeostasis and T cell-independent immune responses.

  • References:
    1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
    2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
    3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
    4. Thompson, J.S. et al. (2001) Science 14:2108.
  • Long Name:
    B cell Activating Factor Receptor
  • Entrez Gene IDs:
    115650 (Human); 72049 (Mouse)
  • Alternate Names:
    BAFF R; BAFFR; BR3; CD268; TNFRSF13C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014;5(0):4092.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
    Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Br. J. Dermatol., 2012;167(2):359-67.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. APRIL Binding to BCMA Activates a JNK2-FOXO3-GADD45 Pathway and Induces a G2/M Cell Growth Arrest in Liver Cells.
    Authors: Notas G, Alexaki V, Kampa M, Pelekanou V, Charalampopoulos I, Sabour-Alaoui S, Pediaditakis I, Dessirier V, Gravanis A, Stathopoulos E, Tsapis A, Castanas E
    J Immunol, 2012;189(10):4748-58.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo.
    Authors: Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin
    PLoS ONE, 2012;7(10):e47361.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Requirement for BAFF and APRIL during B cell development in GALT.
    Authors: Yeramilli VA, Knight KL
    J. Immunol., 2010;184(10):5527-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
    Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
    Blood, 2008;111(5):2744-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007;178(5):2872-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model.
    Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N
    Clin. Cancer Res., 2007;13(19):5903-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
    Authors: Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM
    Blood, 2006;107(7):2882-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
    Authors: Pound JD, Batth BK, Johannessen I, Wood K
    J. Immunol., 2005;174(5):3015-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Decreased survival of B cells of HIV-viremic patients mediated by altered expression of receptors of the TNF superfamily.
    Authors: Moir S, Malaspina A, Pickeral OK, Donoghue ET, Vasquez J, Miller NJ, Krishnan SR, Planta MA, Turney JF, Justement JS, Kottilil S, Dybul M, Mican JM, Kovacs C, Chun TW, Birse CE, Fauci AS
    J. Exp. Med., 2004;200(7):587-99.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 12 Citations
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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