Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 127606
View all Bax Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bax aa 1-171
Accession # Q07812

Specificity

Detects full-length human Bax in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Bax Minus C-Terminus Antibody

Detection of Human Bax Minus C-Terminus antibody by Western Blot.

Detection of Human Bax Minus C-Terminus by Western Blot.

Western blot shows lysate of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Bax antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

Bax in A549 Human Cell Line.

Bax was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human Bax Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Bax Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Bax at approximately 20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Bax by Western Blot

Detection of Human Bax by Western Blot

Effect of B2R and D2R agonists on the expression of pro- and anti-apoptotic proteins.Cells (1.0 × 106) were treated with 100 nM BK, 100 nM SUM, or both agonists simultaneously for 6 or 24 hours. (A) The protein expression levels of Bcl-2, Bcl-xL, and Bax was analyzed by western blotting. The (B) Bcl-2/Bax and (C) Bcl-xL/Bax ratios were calculated by densitometric analysis and the values were normalized to the beta -actin expression level. The figures represent the mean values ± SD from two experiments, compared with the value obtained for the untreated cells, assumed to be 1 (dashed line). &P < 0.001 versus untreated cells, * P < 0.001 versus BK-treated cells, #P < 0.005 versus SUM-treated cells. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30427893), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Bax Minus C-Terminus Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Knockout Validated

Bax is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bax knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line

Reviewed Applications

Read 1 review rated 4 using MAB846 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bax

Bax belongs to the Bcl-2 protein family. It forms homodimers and heterodimers with Bcl-2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Gene Symbol

BAX

UniProt

Q07812

Additional Bax Products

Product Documents for Human Bax Minus C-Terminus Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bax Minus C-Terminus Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human Bax Minus C-Terminus Antibody

Customer Reviews for Human Bax Minus C-Terminus Antibody (1)

4 out of 5
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21697278
    Species: Human
    Verified Customer | Posted 02/25/2015

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Protocols

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