Detection of Human Bax Minus C-Terminus by Western Blot. Western blot shows lysate of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bax belongs to the Bcl-2 protein family. It forms homodimers and
heterodimers with Bcl-2 and functions as an apoptotic activator. Bax
interacts with the mitochondrial voltage dependent anion channel and
promotes the release of cytochrome c.
Bcl Associated X Protein
Entrez Gene IDs:
581 (Human); 12028 (Mouse); 24887 (Rat)
apoptosis regulator BAX; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.