Detection of Human Bax Minus C-Terminus by Western Blot.
Western blot shows lysate of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Bax in A549 Human Cell Line.
Bax was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bax belongs to the Bcl-2 protein family. It forms homodimers and
heterodimers with Bcl-2 and functions as an apoptotic activator. Bax
interacts with the mitochondrial voltage dependent anion channel and
promotes the release of cytochrome c.
Bcl Associated X Protein
Entrez Gene IDs:
581 (Human); 12028 (Mouse); 24887 (Rat)
apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4
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The data collected includes not only links to publications in PubMed,
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