Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Catalog # Availability Size / Price Qty
MAB8560-100
MAB8560-SP
 beta ‑1,3‑Glucuronyltransferase  1/B3GAT1 in Human Brain.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1002707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1
His25-Ile334
Accession # Q9P2W7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry beta ‑1,3‑Glucuronyltransferase  1/B3GAT1 in Human Brain. View Larger

beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in Human Brain. beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB8560) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References
  1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
  2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
  3. Kakuda, S. et al. (2005) Glycobiology 2:203.
  4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
  5. Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name
UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase
Entrez Gene IDs
27087 (Human); 76898 (Mouse); 117108 (Rat)
Alternate Names
B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Beta-1,3-glucuronyltransferase 1; CD57; EC 2.4.1.135; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCATP; GlcAT-P; GLCATPGLCUATP; GLCUATP; glcUAT-P; Glucuronosyltransferase P; HNK1; LEU7; NK1; NK-1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

Reviews for Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

There are currently no reviews for this product. Be the first to review Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody and earn rewards!

Have you used Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review