Human BMP-4 Antibody Summary
Accession # P12644
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
BMP-4 in HepG2 Human Cell Line. BMP-4 was detected in immersion fixed HepG2 human hepatocytoma cell line (positive) or K562 human myelogenous leukemia cell line (negative) using Rabbit Anti-Human BMP-4 Monoclonal Antibody (Catalog # MAB7572) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
BMP-4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1, 5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP-4/BMP-7 disulfidelinked heterodimer (7, 9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13-15).
- Zhang, P. et al. (2008) Blood 111:1933.
- Gambaro, K. et al. 2006, Cell Death Differ. 13:1075.
- Simic, P. and Vukicevic, S. (2005) Cytokine Growth Factor Rev. 16:299.
- Sadlon, T.J. et al. 2004, Stem Cells 22:457.
- Frank, D.B. et al. 2005, Circ. Res. 97:496.
- Wozney, J. et al. (1988) Science 242:1528.
- Cui, Y. et al. (1998) EMBO J. 17:4735.
- Cui, Y. et al. (2001) Genes Dev. 15:2797.
- Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
- Nishimatsu, S. and G.H. Thomsen, (1998) Mech. Dev. 74:75.
- Chen, D. et al. (2004) Growth Factors 22:233.
- Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
- Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
- Jones, C.M. and J.C. Smith, (1998) Dev. Biol. 194:12.
- Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human BMP-4 Antibody
There are currently no reviews for this product. Be the first to review Human BMP-4 Antibody and earn rewards!
Have you used Human BMP-4 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image