Human BMP-6 Antibody Summary
Accession # Q4VBA3
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Human BMP-6 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody. Recombinant Human BMP-6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-6 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-6 Monoclonal Antibody (Catalog # MAB507). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7, and -8 are in the same subgroup.
- Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
- Reddi, A.H. (1998) Nature Biotechnology 16:247.
Citations for Human BMP-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer
Authors: N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti
Sample Types: Whole Cells
TNF? drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling
Authors: LA Hurst, BJ Dunmore, L Long, A Crosby, R Al-Lamki, J Deighton, M Southwood, X Yang, MZ Nikolic, B Herrera, GJ Inman, JR Bradley, AA Rana, PD Upton, NW Morrell
Nat Commun, 2017;8(0):14079.
Sample Types: Whole Cells
Regulation of TMPRSS6 by BMP6 and iron in human cells and mice.
Authors: Meynard D, Vaja V, Sun CC, Corradini E, Chen S, Lopez-Otin C, Grgurevic L, Hong CC, Stirnberg M, Gutschow M, Vukicevic S, Babitt JL, Lin HY
Sample Types: In Vivo
Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
Cancer Res., 2005;65(18):8274-85.
Sample Types: Cell Lysates
Applications: Western Blot
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