Human BNIP3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human BNIP3 by Western Blot.
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Product Details
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Human BNIP3 Antibody Summary

Species Reactivity
Detects human BNIP3 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 670639
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human BNIP3
Accession # Q12983
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BNIP3 antibody by Western Blot. View Larger

Detection of Human BNIP3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with CoCl2.PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human BNIP3 Polyclonal Antibody (Catalog # MAB41471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for BNIP3 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry BNIP3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

BNIP3 in HeLa Human Cell Line. BNIP3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human BNIP3 Polyclonal Antibody (Catalog # MAB41471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNIP3

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.

Long Name
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Entrez Gene IDs
664 (Human); 12176 (Mouse); 84480 (Rat)
Alternate Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kD-interacting protein 3; BNIP3; Nip3

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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Human BNIP3 Antibody
By Anonymous on 06/29/2022
Application: ICC/IF Sample Tested: HeLa human cervical epithelial carcinoma cell line Species: Human