Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 883926
View all c-Maf Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human c-Maf
Met1-Phe372
Accession # O75444

Specificity

Detects human c-Maf in direct ELISA and Western Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human c-Maf Antibody

Detection of Human c-Maf antibody by Western Blot.

Detection of Human c-Maf by Western Blot.

Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Maf Monoclonal Antibody (Catalog # MAB8227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for c-Maf at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c-Maf antibody in RPMI 8226 Human Cell Line by Immunocytochemistry (ICC).

c-Maf in RPMI 8226 Human Cell Line.

c-Maf was detected in immersion fixed RPMI 8226 human multiple myeloma cell line using Mouse Anti-Human c-Maf Monoclonal Antibody (Catalog # MAB8227) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human c-Maf Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed RPMI 8226 human multiple myeloma cell line

Western Blot

2 µg/mL
Sample: RPMI 8226 human multiple myeloma cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB8227 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: c-Maf

c-Maf is an approximately 40 kDa transcriptional regulator that contains one bZIP domain. It can associate into homodimers and heterodimers with other basic leucine zipper transcription factors. c-Maf plays an important role in fetal erythropoiesis, lens development, mechanosensory neuron development, and the differentiation of chondrocytes and osteoblasts. In the pancreas, c-Maf promotes the transcription of glucagon in alpha cells and insulin in beta cells. In immune cells, it controls Th17 and Treg differentiation by regulating the transcription of IL-4, IL-12 p35, IL-21, IL-22, and GM-CSF. Human c-Maf shares 97% amino acid sequence identity with mouse and rat c-Maf. Alternative splicing of human c-Maf generates a long isoform with a 30 aa substitution for the C-terminal methionine.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian)

Alternate Names

CCA4, cMaf, MAF2

Entrez Gene IDs

4094 (Human); 17132 (Mouse); 54267 (Rat)

Gene Symbol

MAF

UniProt

O75444

Additional c-Maf Products

Product Documents for Human c-Maf Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human c-Maf Antibody

For research use only

Citations for Human c-Maf Antibody

Customer Reviews for Human c-Maf Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Human c-Maf Antibody
    Name: Heeseon An
    Application: Western Blot
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/27/2020
    As hc-Maf mostly localizes in the nucleus, prepping nuclear fraction and performing WB might help if the WB signal from the total lysate is too low.
    Human c-Maf Antibody MAB8227
  • Human c-Maf Antibody
    Name: Natalie Erdmann
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: RPMI-8226
    Species: Human
    Verified Customer | Posted 10/24/2016
    ICC/IF on RPMI-8226, JJN3, K-562, KMS12 & Daudi with MAB8227
    Immersion-fixed cells; permeabilized Triton X-100 5 min; blocked 1 hour 10% donkey serum; 1/30 O/N @4oC; Donkey F(ab’)2 anti-mouse IgG (H+L) Alexa Fluor 647 Abcam #ab150103 1 hour at rt; Zeiss AxioImager Cy5 filter cube 500 ms exposure
    Human c-Maf Antibody MAB8227

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Protocols

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FAQs

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Associated Pathways

Th2 Differentiation Pathway Th2 Differentiation Pathway Thumbnail