Human/Canine NGF R/TNFRSF16 Antibody

(4 citations)   
  • Species Reactivity
    Human, Canine
  • Specificity
    Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 74902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
    Lys29-Asn250
    Accession # P08138
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human NGF R/TNFRSF16 Fc Chimera (Catalog # 367-NR)
    under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

NGF R/TNFRSF16 in Human Brain. NGF R/TNFRSF16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

NGF R/TNFRSF16 in Canine Mesenchymal Stem Cells. NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry

NGF R/TNFRSF16 in Human Mesenchymal Stem Cells. NGF R/TNFRSF16 was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NGF R/TNFRSF16
NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3 and Neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).
  • References:
    1. Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
    2. Stemple, D.L. et al. (1992) Cell 71:973.
    3. Morrison, S.J. et al. (1999) Cell 96:737.
    4. Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
    5. Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
    6. Cassiman, D. et al. (2001) Hepatology 33:148.
    7. Long Name:
      Nerve Growth Factor Receptor
    8. Entrez Gene IDs:
      4804 (Human); 18053 (Mouse); 117089 (Rat)
    9. Alternate Names:
      CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    4 Citations: Showing 1 - 4
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    Species
    Applications
    Sample Type
    1. Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream
      Sci Rep, 2016;6(0):32203.
      Species: Mouse
      Sample Type: Whole Tissue
      Application: IHC
    2. Human papillomavirus 16 infection predicts poor outcome in patients with esophageal squamous cell carcinoma.
      Authors: Xi R, Zhang X, Chen X, Pan S, Hui B, Zhang L, Fu S, Li X, Zhang X, Gong T, Guo J, Che S
      Onco Targets Ther, 2015;8(0):573-81.
      Species: Human
      Sample Type: Whole Tissue
      Application: IHC Paraffin-embedded
    3. BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
      Authors: Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
      J. Neuroimmunol., 2008;197(2):128-39.
      Species: Human
      Sample Type: Whole Cells
      Application: ICC
    4. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
      Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
      Leuk. Lymphoma, 2007;48(10):2032-41.
      Species: Human
      Sample Type: Whole Cells
      Application: Flow
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