Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Porcine, Guinea Pig

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 113612
View all CART Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CART
Ala37-Leu116
Accession # Q16568

Specificity

Detects human CART aa 28‑116 in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human CART aa 28‑116 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex)

Western Blot

1 µg/mL
Sample: Recombinant Human CART

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CART

CART, also known as cocaine and amphetamine-related transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations.  In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment.  There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region.  There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist.  These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89).  Human CART is 98% aa identical to both moyuse and rat CART (1-89).  Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions.   In addition, anatomical location is associated with differing lengths of CART.  For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

References

  1. Douglass, J. & S. Daoud (1996) Gene 169:241.
  2. Douglass, J. et al. (1995) J. Neurosci. 15:247.
  3. Adams, L.D. et al. (1999) Brain Res. 848:137.
  4. Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
  5. Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
  6. Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
  7. Thim, L. et al. (1998) FEBS Lett. 428:263.

Long Name

Cocaine- and Amphetamine-regulated Transcript Protein

Alternate Names

CARTPT

Entrez Gene IDs

9607 (Human)

Gene Symbol

CARTPT

UniProt

Q16568

Additional CART Products

Product Documents for Human CART aa 28‑116 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CART aa 28‑116 Antibody

For research use only

Citations for Human CART aa 28‑116 Antibody

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Protocols

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