Human CD31/PECAM-1 Antibody

Detection of CD31 in Human Lymph Node via seqIF™ staining on COMET™ CD31 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Mouse Anti-Human CD31, Monoclonal Antibody (Catalog #MAB11666) at 0.1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​

Detection of Human CD31/PECAM‑1 by Western Blot. Western Blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD31/PECAM‑1 in Human Liver Tumor. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver tumor using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD31/PECAM‑1 in Human Liver. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CD31/PECAM‑1 by Simple Western^TM. Left: Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.5 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 168 kDa (as indicated) using both 5 µg/ml and 25 µg/ml of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) tested at 5 µg/ml (blue line) and 25 µg/ml (green line) in the HUVEC human umbilical vein endothelial cells.