Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Simple Western, COMET

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1094431
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived recombinant human CD31/PECAM01
Gln28-Lys601
Accession # P16284

Specificity

Detects recombinant human CD31/PECAM-1 protein in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD31/PECAM‑1 Antibody

Detection of CD31 in Human Lymph Node via seqIF™ staining on COMET™

CD31 was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Mouse Anti-Human CD31, Monoclonal Antibody (Catalog #MAB11666) at 0.1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​

Detection of Human CD31/PECAM‑1 by Western Blot.

Western Blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD31/PECAM‑1 in Human Liver Tumor.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver tumor using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD31/PECAM‑1 in Human Liver.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CD31/PECAM‑1 by Simple WesternTM.

Left: Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.5 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 168 kDa (as indicated) using both 5 µg/ml and 25 µg/ml of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11666) tested at 5 µg/ml (blue line) and 25 µg/ml (green line) in the HUVEC human umbilical vein endothelial cells.

Applications for Human CD31/PECAM‑1 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

0.05-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver tumor and normal human liver

Multiplex Immunofluorescence

0.1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Lymph Node

Simple Western

5-25 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

UniProt

P16284

Additional CD31/PECAM-1 Products

Product Documents for Human CD31/PECAM‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD31/PECAM‑1 Antibody

For research use only

Customer Reviews for Human CD31/PECAM‑1 Antibody

There are currently no reviews for this product. Be the first to review Human CD31/PECAM‑1 Antibody and earn rewards!

Have you used Human CD31/PECAM‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies