Human CD44s Pan Specific Antibody

Catalog # Availability Size / Price Qty
BBA10
Human CD44s Pan Specific Antibody in Western Blot
3 Images
Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (7)

Human CD44s Pan Specific Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD44s on a panel of CD44 transfected COS cells by flow cytometry (Fox, S.B. et al. (1994) Cancer Res. 54:4539). This antibody recognizes an epitope in the invariant N-terminal region of all CD44 protein isoforms.
Source
Monoclonal Mouse IgG2A Clone # 2C5
Purification
Protein A or G purified from ascites
Immunogen
Recombinant human CD44v3-10 (includes the invariant N-terminal exons and CD44v3-10 exons)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Fox, S.B. et al. (1994) Cancer Res. 54:4539.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human CD44 by Western Blot. View Larger

Detection of Human CD44 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD44 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry CD44 in Human Lymphoma. View Larger

CD44 in Human Lymphoma. CD44 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection  of Human CD44 by Simple WesternTM. View Larger

Detection of Human CD44 by Simple WesternTM. Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 156 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44s, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80-200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83%, and 79% identity with corresponding mouse, rat, equine, canine, and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

References
  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  7. Murakami, D. et al. (2003) Oncogene 22:1511.
  8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

Product Datasheets

Citations for Human CD44s Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. The deubiquitylase OTUB1 mediates ferroptosis via stabilization of SLC7A11
    Authors: T Liu, L Jiang, O Tavana, W Gu
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  2. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions
    Authors: T Murakami, J Kim, Y Li, GE Green, A Shikanov, A Ono
    Nat Commun, 2018;9(1):2436.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
    Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
    Sci Rep, 2018;8(1):420.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Akt signaling is sustained by a CD44 splice isoform-mediated positive feedback loop
    Authors: S Liu, C Cheng
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology
    Authors: M Silva, RK Fung, CB Donnelly, PA Videira, R Sackstein
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  7. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells
    Authors: Brad Dykstra
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  8. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas
    Stem Cell Reports, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Quantitative Characterization of E-selectin Interaction with Native CD44 and P-selectin Glycoprotein Ligand-1 (PSGL-1) Using a Real Time Immunoprecipitation-based Binding Assay.
    Authors: AbuSamra D, Al-Kilani A, Hamdan S, Sakashita K, Gadhoum S, Merzaban J
    J Biol Chem, 2015;290(35):21213-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma.
    Authors: Okada, Toshihir, Nakamura, Teruo, Watanabe, Takayuki, Onoda, Naoyoshi, Ashida, Atsuko, Okuyama, Ryuhei, Ito, Ken-ichi
    PLoS ONE, 2014;9(4):e94487.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
    Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M
    Cytokine, 2014;69(1):47-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
    Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H
    BMC Cancer, 2013;13(0):517.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. KCa3.1 and TRPM7 channels at the uropod regulate migration of activated human T cells.
    Authors: Kuras Z, Yun Y, Chimote A, Neumeier L, Conforti L
    PLoS ONE, 2012;7(8):e43859.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Synovial sarcoma is a stem cell malignancy.
    Authors: Naka N, Takenaka S, Araki N
    Stem Cells, 2010;28(7):1119-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Increased NOS2 predicts poor survival in estrogen receptor-negative breast cancer patients.
    Authors: Glynn SA, Boersma BJ, Dorsey TH, Yi M, Yfantis HG, Ridnour LA, Martin DN, Switzer CH, Hudson RS, Wink DA, Lee DH, Stephens RM, Ambs S
    J. Clin. Invest., 2010;120(11):3843-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009;20(7):1565-76.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Anticancer chemotherapy inhibits MHC class I-related chain a ectodomain shedding by downregulating ADAM10 expression in hepatocellular carcinoma.
    Authors: Kohga K, Takehara T, Tatsumi T, Miyagi T, Ishida H, Ohkawa K, Kanto T, Hiramatsu N, Hayashi N
    Cancer Res., 2009;69(20):8050-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Cortactin underpins CD44-promoted invasion and adhesion of breast cancer cells to bone marrow endothelial cells.
    Authors: Hill A, McFarlane S, Mulligan K, Gillespie H, Draffin JE, Trimble A, Ouhtit A, Johnston PG, Harkin DP, McCormick D, Waugh DJ
    Oncogene, 2006;25(45):6079-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. CD44 potentiates the adherence of metastatic prostate and breast cancer cells to bone marrow endothelial cells.
    Authors: Draffin JE, McFarlane S, Hill A, Johnston PG, Waugh DJ
    Cancer Res., 2004;64(16):5702-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells.
    Authors: Inada E, Saitoh I, Watanabe S, Aoki R, Miura H, Ohtsuka M, Murakami T, Sawami T, Yamasaki Y, Sato M
    Int J Oral Sci, 0;7(3):144-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

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Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD44s Pan Specific Antibody

Average Rating: 4.6 (Based on 7 Reviews)

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Human CD44s Pan Specific Antibody
By Anonymous on 06/12/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human CD44s Pan Specific Antibody
By Anonymous on 01/30/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

MAB7045 was used as the capture antibody along with BBA10 as the detection antibody. Recombinant human CD44 Fc Chimera (3660-CD) was used as the calibrator material. Human serum and plasma samples were diluted 1:5 and all were quantifiable. Parallelism looked good.


Human CD44s Pan Specific Antibody
By Anonymous on 01/19/2019
Application: IHC for UMUC with one control 2 condtion Sample Tested: Umbilical vein endothelial cells Species: Human

Human CD44s Pan Specific Antibody
By Anonymous on 01/18/2019
Application: Chondroitiase treated increase CD44 when co-culture with macrophage Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Human CD44s Pan Specific Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human CD44s Pan Specific Antibody
By Anonymous on 06/28/2017
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human

Human CD44s Pan Specific Antibody
By Anonymous on 12/04/2016
Application: WB Sample Tested: Cell Lysates Species: Human

Protein expression confirms sorting results. Antibody is sensitive and reliable across multiple cell lines.