Human CD44s Pan Specific Antibody

(16 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD44s on a panel of CD44 transfected COS cells by flow cytometry (Fox, S.B. et al. (1994) Cancer Res. 54:4539). This antibody recognizes an epitope in the invariant N-terminal region of all CD44 protein isoforms.
  • Source
    Monoclonal Mouse IgG2A Clone # 2C5
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Recombinant human CD44v3-10 (includes the invariant N-terminal exons and CD44v3-10 exons)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood monocytes
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Fox, S.B. et al. (1994) Cancer Res. 54:4539.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD44 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD44 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
CD44 in Human Lymphoma. CD44 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human CD44 by Simple WesternTM. Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 156 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44s, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80-200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83%, and 79% identity with corresponding mouse, rat, equine, canine, and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

  • References:
    1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
    2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
    3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
    4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
    5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
    6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
    7. Murakami, D. et al. (2003) Oncogene 22:1511.
    8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
  • Entrez Gene IDs:
    960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
  • Alternate Names:
    CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology
    Authors: M Silva, RK Fung, CB Donnelly, PA Videira, R Sackstein
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  2. Akt signaling is sustained by a CD44 splice isoform-mediated positive feedback loop
    Authors: S Liu, C Cheng
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells
    Authors: Brad Dykstra
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  4. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas
    Stem Cell Reports, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Quantitative Characterization of E-selectin Interaction with Native CD44 and P-selectin Glycoprotein Ligand-1 (PSGL-1) Using a Real Time Immunoprecipitation-based Binding Assay.
    Authors: AbuSamra D, Al-Kilani A, Hamdan S, Sakashita K, Gadhoum S, Merzaban J
    J Biol Chem, 2015;290(35):21213-30.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
    Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M
    Cytokine, 2014;69(1):47-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma.
    Authors: Okada, Toshihir, Nakamura, Teruo, Watanabe, Takayuki, Onoda, Naoyoshi, Ashida, Atsuko, Okuyama, Ryuhei, Ito, Ken-ichi
    PLoS ONE, 2014;9(4):e94487.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  8. CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
    Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H
    BMC Cancer, 2013;13(0):517.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. KCa3.1 and TRPM7 channels at the uropod regulate migration of activated human T cells.
    Authors: Kuras Z, Yun Y, Chimote A, Neumeier L, Conforti L
    PLoS ONE, 2012;7(8):e43859.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Synovial sarcoma is a stem cell malignancy.
    Authors: Naka N, Takenaka S, Araki N
    Stem Cells, 2010;28(7):1119-31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Increased NOS2 predicts poor survival in estrogen receptor-negative breast cancer patients.
    Authors: Glynn SA, Boersma BJ, Dorsey TH, Yi M, Yfantis HG, Ridnour LA, Martin DN, Switzer CH, Hudson RS, Wink DA, Lee DH, Stephens RM, Ambs S
    J. Clin. Invest., 2010;120(11):3843-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Anticancer chemotherapy inhibits MHC class I-related chain a ectodomain shedding by downregulating ADAM10 expression in hepatocellular carcinoma.
    Authors: Kohga K, Takehara T, Tatsumi T, Miyagi T, Ishida H, Ohkawa K, Kanto T, Hiramatsu N, Hayashi N
    Cancer Res., 2009;69(20):8050-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  13. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009;20(7):1565-76.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Cortactin underpins CD44-promoted invasion and adhesion of breast cancer cells to bone marrow endothelial cells.
    Authors: Hill A, McFarlane S, Mulligan K, Gillespie H, Draffin JE, Trimble A, Ouhtit A, Johnston PG, Harkin DP, McCormick D, Waugh DJ
    Oncogene, 2006;25(45):6079-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. CD44 potentiates the adherence of metastatic prostate and breast cancer cells to bone marrow endothelial cells.
    Authors: Draffin JE, McFarlane S, Hill A, Johnston PG, Waugh DJ
    Cancer Res., 2004;64(16):5702-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 16 Citations
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 WB Human Anonymous 06/28/2017
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Summary

ApplicationWestern Blot
Sample TestedA549 human lung carcinoma cell line
SpeciesHuman
Western Blot Human CD44s Pan Specific Antibody BBA10
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Excellent
 WB Human Anonymous 12/04/2016
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Western Blot Human CD44s Pan Specific Antibody BBA10
Western Blot: Human CD44s Pan Specific Antibody [BBA10]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsProtein expression confirms sorting results. Antibody is sensitive and reliable across multiple cell lines.

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