Human CD68/SR-D1 Antibody

Catalog # Availability Size / Price Qty
MAB20401
MAB20401-SP
CD68/SR-D1 in Human PBMCs.
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Product Details
Citations (16)
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Supplemental Products
Reviews (1)

Human CD68/SR-D1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD68/SR‑D1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 298807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
U937 human histiocytic lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD68/SR-D1 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD68/SR-D1 in Human PBMCs. CD68/SR-D1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Human CD68/SR-D1 Monoclonal Antibody (Catalog # MAB20401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Entrez Gene IDs
968 (Human); 12514 (Mouse); 287435 (Rat)
Alternate Names
CD_antigen: CD68; CD68 antigen; CD68 molecule; CD68; DKFZp686M18236; gp110; LAMP4; macrophage antigen CD68; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1

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Citations for Human CD68/SR-D1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Impact of Enzymatically Extracted High Molecular Weight Fucoidan on Lipopolysaccharide-Induced Endothelial Activation and Leukocyte Adhesion
    Authors: Kirsten, N;Ohmes, J;Mikkelsen, MD;Nguyen, TT;Blümel, M;Wang, F;Tasdemir, D;Seekamp, A;Meyer, AS;Fuchs, S;
    Marine drugs
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. CAGE sequencing reveals CFTR-dependent dysregulation of type I IFN signaling in activated cystic fibrosis macrophages
    Authors: Gillan, JL;Chokshi, M;Hardisty, GR;Clohisey Hendry, S;Prasca-Chamorro, D;Robinson, NJ;Lasota, B;Clark, R;Murphy, L;Whyte, MKB;Baillie, JK;Davidson, DJ;Bao, G;Gray, RD;
    Science advances
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo
    Authors: F Real, A Zhu, B Huang, A Belmellat, A Sennepin, T Vogl, C Ransy, M Revol, R Arrigucci, A Lombès, J Roth, ML Gennaro, F Bouillaud, S Cristofari, M Bomsel
    Nature Communications, 2022-10-11;13(1):5956.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. The interplay between the airway epithelium and tissue macrophages during the SARS-CoV-2 infection
    Authors: Emilia Barreto-Duran, Artur Szczepański, Adrianna Gałuszka-Bulaga, Marcin Surmiak, Maciej Siedlar, Marek Sanak et al.
    Frontiers in Immunology
  5. Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection
    Authors: RM Gopalakris, M Aid, NB Mercado, C Davis, S Malik, E Geiger, V Varner, R Jones, SE Bosinger, C Piedra-Mor, AJ Martinot, DH Barouch, RK Reeves, CS Tan
    JCI Insight, 2021-10-22;6(20):.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  6. Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response
    Authors: Z Feng, J Zhou, Y Liu, R Xia, Q Li, L Yan, Q Chen, X Chen, Y Jiang, G Chao, M Wang, G Zhou, Y Zhang, Y Wang, H Xia
    Cell Death and Differentiation, 2021-03-22;0(0):.
    Species: Human, Mouse
    Sample Types: Protein
    Applications: ELISA Control
  7. Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis
    Authors: Liping Qi, Kui Wu, Shutian Shi, Qingwei Ji, Huangtai Miao, Que Bin
    Experimental and Therapeutic Medicine
  8. Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression
    Authors: P Zhao, B Wang, Z Zhang, W Zhang, Y Liu
    Cell Death Dis, 2019-10-10;10(10):776.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Bordetella Adenylate Cyclase Toxin Inhibits Monocyte-to-Macrophage Transition and Dedifferentiates Human Alveolar Macrophages into Monocyte-like Cells
    Authors: JN Ahmad, J Holubova, O Benada, O Kofronova, L Stehlik, M Vasakova, P Sebo
    MBio, 2019-09-24;10(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  10. Impact of heat therapy on recovery after eccentric exercise in humans
    Authors: Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini
    Journal of Applied Physiology
  11. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization
    Authors: BJ Silva, MG Barbosa, PR Andrade, H Ferreira, JA Nery, S C“rte-Real, GM da Silva, PS Rosa, M Fabri, EN Sarno, RO Pinheiro
    PLoS Pathog, 2017-01-05;13(1):e1006103.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014-06-11;68(9):1029-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009-10-28;1(4):4ra10.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  14. Knockdown of Stat3 activity in vivo prevents diabetic glomerulopathy.
    Authors: Lu TC, Wang ZH, Feng X, Chuang PY, Fang W, Shen Y, Levy DE, Xiong H, Chen N, He JC
    Kidney Int., 2009-04-08;76(1):63-71.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity.
    Authors: Lumeng CN, Deyoung SM, Bodzin JL, Saltiel AR
    Diabetes, 2007-01-01;56(1):16-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Human periprosthetic tissues implanted in severe combined immunodeficient mice respond to gene transfer of a cytokine inhibitor.
    Authors: Yang SY, Nasser S, Markel DC, Robbins PD, Wooley PH
    J Bone Joint Surg Am, 2005-05-01;87(5):1088-97.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human CD68/SR-D1 Antibody

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Human CD68/SR-D1 Antibody
By Anonymous on 05/03/2017
Application: IHC Sample Tested: Adult lung Species: Human