Human CD68/SR-D1 Antibody MAB20401: R&D Systems

Human CD68/SR-D1 Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD68/SR‑D1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 298807
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
    Asn22-Ile320
    Accession # AAB25811
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    U937 human histiocytic lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD68/SR-D1 in Human PBMCs. CD68/SR-D1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Human CD68/SR-D1 Monoclonal Antibody (Catalog # MAB20401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD68/SR-D1
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.
  • Entrez Gene IDs:
    968 (Human)
  • Alternate Names:
    CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization
    Authors: BJ Silva, MG Barbosa, PR Andrade, H Ferreira, JA Nery, S C“rte-Real, GM da Silva, PS Rosa, M Fabri, EN Sarno, RO Pinheiro
    PLoS Pathog, 2017;13(1):e1006103.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014;68(9):1029-35.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  3. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009;1(4):4ra10.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Knockdown of Stat3 activity in vivo prevents diabetic glomerulopathy.
    Authors: Lu TC, Wang ZH, Feng X, Chuang PY, Fang W, Shen Y, Levy DE, Xiong H, Chen N, He JC
    Kidney Int., 2009;76(1):63-71.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity.
    Authors: Lumeng CN, Deyoung SM, Bodzin JL, Saltiel AR
    Diabetes, 2007;56(1):16-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. Human periprosthetic tissues implanted in severe combined immunodeficient mice respond to gene transfer of a cytokine inhibitor.
    Authors: Yang SY, Nasser S, Markel DC, Robbins PD, Wooley PH
    J Bone Joint Surg Am, 2005;87(5):1088-97.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Isotype Controls
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Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human CD68/SR-D1 Antibody MAB20401
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Excellent
 IHC Human Anonymous 05/03/2017
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Immunohistochemistry Human CD68/SR-D1 Antibody MAB20401
Immunohistochemistry: Human CD68/SR-D1 Antibody [MAB20401]

Summary

ApplicationImmunohistochemistry
Sample TestedAdult lung
SpeciesHuman

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