Human CD68/SR‑D1 Antibody

R&D Systems | Catalog # MAB20401

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, ELISA Control, IHC-

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 298807
View all CD68/SR-D1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811

Specificity

Detects human CD68/SR‑D1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD68/SR‑D1 Antibody

Detection of CD68/SR‑D1 in Human Liver.

CD68/SR‑D1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD68/SR‑D1 Monoclonal Antibody (Catalog # MAB20401) at 0.1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD68/SR‑D1 Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB20401 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Gene Symbol

CD68

UniProt

AAB25811

Additional CD68/SR-D1 Products

Product Documents for Human CD68/SR‑D1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD68/SR‑D1 Antibody

For research use only

Citations for Human CD68/SR‑D1 Antibody

Customer Reviews for Human CD68/SR‑D1 Antibody (1)

5 out of 5
1 Customer Rating
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  • Human CD68/SR-D1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 05/03/2017
    Human CD68/SR‑D1 Antibody MAB20401

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Protocols

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FAQs

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