Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 454729
View all CD81 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human CD81
Accession # P60033

Specificity

Detects human CD81 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD81 MAb (Clone 454729)

CD81 antibody in Jurkat human acute T cell leukemia cell line by Immunocytochemistry (ICC).

CD81 in Jurkat human acute T cell leukemia cell line.

CD81 was detected in immersion fixed Jurkat human acute T cell leukemia cell line (left panel; positive staining) and HepG2 human hepatocellular carcinoma cell line (right panel; negative staining) using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46151) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD81 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

CD81 in Human Tonsil.

CD81 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46151) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD81 MAb (Clone 454729)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Reviewed Applications

Read 2 reviews rated 5 using MAB46151 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD81

CD81, also known as TAPA-1 and TM4SF4, is a widely expressed protein in the tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Human CD81 shares 92%‑93% aa sequence identity with mouse and rat CD81.

Alternate Names

CD81, TAPA-1, TSPAN28

Entrez Gene IDs

975 (Human); 12520 (Mouse); 25621 (Rat)

Gene Symbol

CD81

UniProt

P60033

Additional CD81 Products

Product Documents for Human CD81 MAb (Clone 454729)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD81 MAb (Clone 454729)

For research use only

Customer Reviews for Human CD81 MAb (Clone 454729) (2)

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  • Human CD81 MAb (Clone 454729)
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 07/25/2022
    Human CD81 MAb (Clone 454729) MAB46151
  • Human CD81 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 12/08/2021
    Human CD81 MAb (Clone 454729) MAB46151

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Protocols

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