Human CEBP  alpha Antibody

Catalog # Availability Size / Price Qty
MAB7094
MAB7094-SP
Detection of Human CEBP  alpha  by Western Blot.
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Product Details
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Reviews (2)

Human CEBP  alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEBP alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CEBP beta, gamma, delta, epsilon, or zeta is observed.
Source
Monoclonal Mouse IgG2B Clone # 692716
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CEBP alpha
Met1-Ala124
Accession # P49715
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CEBP a antibody by Western Blot. View Larger

Detection of Human CEBP alpha by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CEBP a antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

CEBP alpha in Human Mesenchymal Stem Cells. CEBP a was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEBP alpha

CCAAT-enhancer binding protein alpha (CEBP alpha ) is a widely expressed 42 kDa basic-leucine zipper transcription factor that forms functional homodimers and heterodimers with CEBP beta and CEBP gamma. CEBP alpha inhibits mitotic progression through multiple mechanisms and also promotes terminal differentiation of granulocytes and adipocytes. Its activity is regulated by SUMOylation and by phosphorylation at multiple serine residues. CEBP alpha contains three TE transactivation domains (aa 1‑69, aa 70-96, and aa 126-200) and one basic DNA binding motif and leucine zipper (aa 286 - 345). A 30 kDa isoform is generated by the use of an internal translation initiation site. The p30 isoform functions as a dominant negative factor by heterodimerizing with and inhibiting full length CEBP alpha activity. Within aa 1‑124, human CEBP alpha shares 93% amino acid sequence identity with mouse and rat CEBP alpha, respectively.

Long Name
CCAAT/enhancer-Binding Protein alpha
Entrez Gene IDs
1050 (Human); 12606 (Mouse); 24252 (Rat)
Alternate Names
C/EBP alpha; C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP alpha; CEBP; CEBPA

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CEBP  alpha Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human CEBP  alpha Antibody
By Anonymous on 12/27/2021
Application: ICC/IF Sample Tested: Lung fibroblasts Species: Human

Human CEBP  alpha Antibody
By Anonymous on 10/25/2019
Application: WB Sample Tested: Adipose tissue Species: Mouse