Detection of Human CEBP alpha by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CEBP alpha Monoclonal Antibody (Catalog # MAB7094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP alpha at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CEBP alpha in Human Mesenchymal Stem Cells. CEBP alpha was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human CEBP alpha Monoclonal Antibody (Catalog # MAB7094) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEBP alpha
CCAAT-enhancer binding protein alpha (CEBP alpha ) is a widely expressed 42 kDa basic-leucine zipper transcription factor that forms functional homodimers and heterodimers with CEBP beta and CEBP gamma. CEBP alpha inhibits mitotic progression through multiple mechanisms and also promotes terminal differentiation of granulocytes and adipocytes. Its activity is regulated by SUMOylation and by phosphorylation at multiple serine residues. CEBP alpha contains three TE transactivation domains (aa 1‑69, aa 70-96, and aa 126-200) and one basic DNA binding motif and leucine zipper (aa 286 - 345). A 30 kDa isoform is generated by the use of an internal translation initiation site. The p30 isoform functions as a dominant negative factor by heterodimerizing with and inhibiting full length CEBP alpha activity. Within aa 1‑124, human CEBP alpha shares 93% amino acid sequence identity with mouse and rat CEBP alpha, respectively.
CCAAT/enhancer-Binding Protein alpha
Entrez Gene IDs:
1050 (Human); 12606 (Mouse); 24252 (Rat)
C/EBP alpha; C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP; CEBPA