Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 422818
View all Chymase/CMA1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1
Gly20-Asn247
Accession # P23946

Specificity

Detects human Chymase/CMA1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) MCPT-1, rmMCPT-6, rmMCPT-7, or rmMCPT-11.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Chymase/CMA1 Antibody

Detection of Human Chymase/CMA1 antibody by Western Blot.

Detection of Human Chymase/CMA1 by Western Blot.

Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human Chymase/CMA1 ELISA Standard Curve.

Recombinant Human Chymase/CMA1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (MAB40991) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human Chymase/CMA1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB40991).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Chymase/CMA1 DuoSet ELISA Kit (Catalog # DY4099-05) for convenient development of a sandwich ELISA.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Chymase/CMA1 (Catalog # 4099-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human small intestine tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB4099 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chymase/CMA1

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2‑residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References

  1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
  2. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
  3. Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
  4. Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.

Alternate Names

CMA1, CYH, MCP3P, Mcpt5, MCT1

Entrez Gene IDs

1215 (Human); 17228 (Mouse); 25627 (Rat)

Gene Symbol

CMA1

UniProt

P23946

Additional Chymase/CMA1 Products

Product Documents for Human Chymase/CMA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Chymase/CMA1 Antibody

For research use only

Citations for Human Chymase/CMA1 Antibody

Customer Reviews for Human Chymase/CMA1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22180785
    Species: Human
    Verified Customer | Posted 02/20/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23312967
    Species: Human
    Verified Customer | Posted 02/20/2015

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