Human Coagulation Factor V Antibody Summary
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Coagulation Factor V ELISA Standard Curve. Recombinant Human Coagulation Factor V protein was serially diluted 2-fold and captured by Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor V
Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.
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