Human Coagulation Factor V Antibody

Catalog # Availability Size / Price Qty
MAB98562-100
MAB98562-SP
Human  Coagulation Factor V ELISA Standard Curve.
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Human Coagulation Factor V Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor V in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 986237
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Coagulation Factor V synthetic peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Coagulation Factor V ELISA Standard Curve. Recombinant Human Coagulation Factor V protein was serially diluted 2-fold and captured by Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor V

Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.

Long Name
Coagulation Factor V
Entrez Gene IDs
2153 (Human); 14067 (Mouse); 304929 (Rat)
Alternate Names
Activated protein C cofactor; Cf5; Cf-5; Coagulation Factor 5; coagulation factor V (proaccelerin, labile factor); coagulation factor V jinjiang A2 domain; Coagulation Factor V; F5; factor V Leiden; Factor V; FVL; PCCF; Proaccelerin, Labile Factor; RPRGL1; THPH2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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