Human COMMD1 Antibody

Name: Human COMMD1 Antibody
Brand: R&D Systems
SKU: MAB7526-SP
Rating: 5 (1 reviews)

MAB7526 2 Citations 1 Review Datasheet / COA / SDS

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MAB7526
MAB7526-SP

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Detection of Human COMMD1 by Western Blot.

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Citations (2)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human COMMD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human COMMD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Attractin or rhCaspr1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 762203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human COMMD1
Ser37-Ser135
Accession # Q8N668

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human COMMD1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, HepG2 human hepatocellular carcinoma cell line, and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for COMMD1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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COMMD1 in U2OS Human Cell Line. COMMD1 was detected in immersion fixed U2OS human osteosarcoma cell line using Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMMD1

COMMD1, also known as MURR1 and the copper toxicosis gene product, is a ubiquitously expressed 21 kDa copper binding protein. It contains an N-terminal domain (aa 1-121) and C-terminal domain (aa 125-190) which are separated by a protease sensitive site. It forms high molecular weight oligomeric complexes both in solution and in association with phospholipid membranes. COMMD1 is found in the nucleus and cytoplasm as well as in endocytic vesicle membrane fractions. It binds and regulates the activity of a variety of proteins including the copper transporter Wilson disease protein (ATP7B), the delta ENaC epithelial cell sodium channel, the cystic fibrosis transmembrane regulator (CFTR), superoxide dismutase 1 (SOD1), XIAP, HIF-1 alpha, and Cullin RING ubiquitin ligases. COMMD1 promotes the ubiquitination and degradation of the RelA subunit of NFkB, thereby inhibiting its chromatin association and nuclear targeting as well as the replication of HIV in resting T cells. Within aa 37-135, human COMMD1 shares 94% aa sequence identity with mouse and rat COMMD1.

Long Name

Copper Metabolism [MURR1] Domain Containing 1

Entrez Gene IDs

150684 (Human); 17846 (Mouse)

Alternate Names

C2orf5; COMM domain-containing protein 1; COMMD1; copper metabolism (Murr1) domain containing 1; copper metabolism gene MURR1; MGC27155; MURR1; MURR1chromosome 2 open reading frame 5 (MURR1); Protein Murr1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human COMMD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking
Authors: CG Campion, K Zaoui, T Verissimo, S Cossette, H Matsuda, N Solban, P Hamet, J Tremblay
Cell Rep, 2018;24(3):670-684.e7.
Species: Human
Sample Types: Whole Cells
Applications: ICC
COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking
Authors: A Fedoseienk, M Wijers, JC Wolters, D Dekker, M Smit, N Huijkman, N Kloosterhu, H Klug, A Schepers, K Willems va, JH Levels, DD Billadeau, MH Hofker, J van Deurse, M Westerterp, E Burstein, JA Kuivenhove, B van de Slu
Circ. Res., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation