Human COMMD1 Antibody Summary
Ser37-Ser135
Accession # Q8N668
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human COMMD1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, HepG2 human hepatocellular carcinoma cell line, and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for COMMD1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

COMMD1 in U2OS Human Cell Line. COMMD1 was detected in immersion fixed U2OS human osteosarcoma cell line using Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COMMD1
COMMD1, also known as MURR1 and the copper toxicosis gene product, is a ubiquitously expressed 21 kDa copper binding protein. It contains an N-terminal domain (aa 1-121) and C-terminal domain (aa 125-190) which are separated by a protease sensitive site. It forms high molecular weight oligomeric complexes both in solution and in association with phospholipid membranes. COMMD1 is found in the nucleus and cytoplasm as well as in endocytic vesicle membrane fractions. It binds and regulates the activity of a variety of proteins including the copper transporter Wilson disease protein (ATP7B), the delta ENaC epithelial cell sodium channel, the cystic fibrosis transmembrane regulator (CFTR), superoxide dismutase 1 (SOD1), XIAP, HIF-1 alpha, and Cullin RING ubiquitin ligases. COMMD1 promotes the ubiquitination and degradation of the RelA subunit of NFkB, thereby inhibiting its chromatin association and nuclear targeting as well as the replication of HIV in resting T cells. Within aa 37-135, human COMMD1 shares 94% aa sequence identity with mouse and rat COMMD1.
Product Datasheets
Citations for Human COMMD1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking
Authors: CG Campion, K Zaoui, T Verissimo, S Cossette, H Matsuda, N Solban, P Hamet, J Tremblay
Cell Rep, 2018;24(3):670-684.e7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking
Authors: A Fedoseienk, M Wijers, JC Wolters, D Dekker, M Smit, N Huijkman, N Kloosterhu, H Klug, A Schepers, K Willems va, JH Levels, DD Billadeau, MH Hofker, J van Deurse, M Westerterp, E Burstein, JA Kuivenhove, B van de Slu
Circ. Res., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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