Human COMMD1 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human COMMD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Attractin or rhCaspr1 is observed.
• Source
  Monoclonal Mouse IgG_2B Clone # 762203
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant human COMMD1
  Ser37-Ser135
  Accession # Q8N668
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.2 µg/mL
  See below
• Immunocytochemistry
  8-25 µg/mL
  See below

Western Blot click image to view larger

Detection of Human COMMD1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, HepG2 human hepatocellular carcinoma cell line, and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for COMMD1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry click image to view larger

COMMD1 in U2OS Human Cell Line. COMMD1 was detected in immersion fixed U2OS human osteosarcoma cell line using Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Sterile PBS to a final concentration of 0.5 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Copper Metabolism [MURR1] Domain Containing 1

• Entrez Gene IDs:
  150684 (Human); 17846 (Mouse)

• Alternate Names:
  C2orf5; COMM domain-containing protein 1; COMMD1; copper metabolism (Murr1) domain containing 1; copper metabolism gene MURR1; MGC27155; MURR1; MURR1chromosome 2 open reading frame 5 (MURR1); Protein Murr1

FAQs

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