Human CRLR Antibody Summary
Accession # Q16602
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CRLR in KG-1 Human Cell Line. CRLR was detected in immersion fixed KG‑1 human acute myelogenous leukemia cell line using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CRLR in Human Lung. CRLR was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The calcitonin receptor-like receptor (CRLR), a class B GPCR, forms a heterodimer with receptor activity-modifying protein 2 (RAMP2), and serves as the adrenomedullin (AM) receptor to control neovascularization, while CRLR and RAMP1 form the calcitonin gene-related peptide (CGRP) receptor. CALCRL is pharmacologically identical to CGRP receptor-1, a putative molecular target of CGRP and adrenomedullin. CALCRL is predominantly expressed in the lung and heart.
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Cell and Tissue Staining Kits
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