Human CRLR Antibody

Catalog # Availability Size / Price Qty
MAB10044-100
MAB10044-SP
CRLR in KG-1 Human Cell Line.
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Human CRLR Antibody Summary

Species Reactivity
Human
Specificity
Detects human CALCRL in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 998820
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human CALCRL
Accession # Q16602
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry CRLR antibody in KG-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

CRLR in KG-1 Human Cell Line. CRLR was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CRLR antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

CRLR in Human Lung. CRLR was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRLR

The calcitonin receptor-like receptor (CRLR), a class B GPCR, forms a heterodimer with receptor activity-modifying protein 2 (RAMP2), and serves as the adrenomedullin (AM) receptor to control neovascularization, while CRLR and RAMP1 form the calcitonin gene-related peptide (CGRP) receptor. CALCRL is pharmacologically identical to CGRP receptor-1, a putative molecular target of CGRP and adrenomedullin. CALCRL is predominantly expressed in the lung and heart.

Long Name
Calcitonin Receptor-like Receptor
Entrez Gene IDs
10203 (Human); 54598 (Mouse); 25029 (Rat)
Alternate Names
calcitonin gene-related peptide type 1 receptor; calcitonin receptor-like; CALCRL; CGRP type 1 receptor; CGRPR; CGRPRCRLRCalcitonin receptor-like receptor; CRLR

Product Datasheets

FAQs

  1. What region of the protein was the synthetic peptide immunogen derived for Catalog # MAB10044 (Human CRLR antibody)?

    • The synthetic peptide immunogen for Catalog # MAB10044 is derived from the 1-50 aa N-terminal extracellular region.

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CRLR Antibody

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Human CRLR Antibody
By Anonymous on 02/23/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: KG-1 Human Cell Line Species: Human