Human Cytokeratin 7 Antibody

Detection of Human Cytokeratin 7 by Western Blot. Western Blot shows lysates of KATO‑III human gastric carcinoma cell line, OVCAR‑3 human ovarian carcinoma cell line, SK‑BR‑3 human breast cancer cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cytokeratin 7 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Cytokeratin 7 in Human Lung Cancer. Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11626) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Cytokeratin 7 in Human Placenta. Cytokeratin 7 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11626) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Cytokeratin 7 in SK‑BR‑3 Human Cell Line. Cytokeratin 7 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Mouse Anti-Human Cytokeratin 7 Monoclonal Antibody (Catalog # MAB11626) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.