Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot.
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Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody Summary

Species Reactivity
Detects human Cytosolic Sulfotransferase 4A1/SULT4A1 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, rhSULT2A1, or rhSULT2B1 is observed.
Monoclonal Mouse IgG2B Clone # 634630
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Cytosolic Sulfotransferase 4A1/SULT4A1
Accession # Q9BR01
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 antibody by Western Blot. View Larger

Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cytosolic Sulfotransferase 4A1/SULT4A1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Cytosolic Sulfotransferase 4A1/SULT4A1 in Human Brain. Cytosolic Sulfotransferase 4A1/SULT4A1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 4A1/SULT4A1

Cytosolic SULT4A1 (Sulfotransferase 4A1 also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes.  SULT4A1 message is widely expressed.  However, it appears to be translated only in brain.  SULT4A is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates.  Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation.  Human SULT4A1 is 284 amino acids (aa) in length.  A weak PAPS binding site is found between aa 246-254.  There are two potential isoforms, one that shows a 13 aa substitution for aa 248-284, and a second that contains a 48 aa substitution for aa 202-247.  Full length human SULT4A1 is 98% aa identical to mouse SULT4A1.

Entrez Gene IDs
25830 (Human); 29859 (Mouse)
Alternate Names
Brain sulfotransferase-like protein; BRSTL1; BR-STL-1; Cytosolic Sulfotransferase 4A1; EC 2.8.2.-; hBR-STL; hBR-STL-1MGC40032; nervous system cytosolic sulfotransferase; Nervous system sulfotransferase; NST; ST4A1; sulfotransferase 4A1; sulfotransferase family 4A, member 1; sulfotransferase-related protein; SULT4A1; SULTX3; SULTX3DJ388M5.3

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Isotype Controls

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