Human DC-STAMP Antibody

Catalog # Availability Size / Price Qty
MAB7824
MAB7824-SP
Detection of DC-STAMP in Human Mature Dendritic Cells by Flow Cytometry.
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Human DC-STAMP Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC-STAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DC-STAMP is observed.
Source
Monoclonal Mouse IgG2B Clone # 788524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DC-STAMP
Asp314-Thr376
Accession # Q9H295
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of DC-STAMP antibody in Human Mature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of DC-STAMP in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells derived from CD14+Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours were stained with Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F) and either (A) Mouse IgG2BIsotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human DC-STAMP Monoclonal Antibody (Catalog # MAB7824) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-STAMP

DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.

Long Name
Dendritic Cell-specific Transmembrane Protein
Entrez Gene IDs
81501 (Human); 75766 (Mouse); 299889 (Rat)
Alternate Names
DCSTAMP; DC-STAMP; dendritic cell-specific transmembrane protein; FIND; IL-4-induced Protein; TM7SF4; transmembrane 7 superfamily member 4

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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