Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 251123
View all DLL1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DLL1
Ser22-Gly540
Accession # AAG09716

Specificity

Detects human DLL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DLL3 or rhDLL4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DLL1 Antibody

DLL1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

DLL1 in HepG2 Human Cell Line.

DLL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

DLL1 in Human Breast Cancer Tissue.

DLL1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of DLL1 by Immunohistochemistry

Detection of DLL1 by Immunohistochemistry

Expression pattern of DLL1 in the developing cochlea of primates. (a) In E87 cochlea, no expression of DLL1 was detected in either ATOH1-positive hair cells or SOX2-positive supporting cells. (b) In contrast, a relatively weak expression of DLL1 was observed in ATOH1-positive hair cells in the E92 cochlea. (c) In E96 cochlea, the expression of DLL1 in hair cells became more pronounced. (d–h) After E101, DLL1 was continuously expressed in hair cells. Nuclei were counterstained with Hoechst (blue). Scale bar: 20 μm. IHC, inner hair cell; OHC, the outer hair cell. (a,b,d,g,h): basal turn, (c,e,f): middle turn. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37351521), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of DLL1 by Immunohistochemistry

Detection of DLL1 by Immunohistochemistry

Expression pattern of DLL1 in the developing cochlea of primates. (a) In E87 cochlea, no expression of DLL1 was detected in either ATOH1-positive hair cells or SOX2-positive supporting cells. (b) In contrast, a relatively weak expression of DLL1 was observed in ATOH1-positive hair cells in the E92 cochlea. (c) In E96 cochlea, the expression of DLL1 in hair cells became more pronounced. (d–h) After E101, DLL1 was continuously expressed in hair cells. Nuclei were counterstained with Hoechst (blue). Scale bar: 20 μm. IHC, inner hair cell; OHC, the outer hair cell. (a,b,d,g,h): basal turn, (c,e,f): middle turn. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37351521), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human DLL1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

1.7-25 µg/mL
Sample: immersion fixed paraffin-embedded sections of human breast cancer tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90‑100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6‑11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References

  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
  3. Karanu, F.N. et al. (2001) Blood 97:1960. 
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
  6. Takahashi, Y. et al. (2003) Development 130:4259. 
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

AAG09716

Additional DLL1 Products

Product Documents for Human DLL1 Antibody

Certificate of Analysis

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Product Specific Notices for Human DLL1 Antibody

For research use only

Related Research Areas

Notch Pathway
Somite Markers

Citations for Human DLL1 Antibody

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Protocols

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