|Detection of Human EGFR (aa 746-750 deletion) by Western Blot. Western blot shows lysates of HCC827 human non-small cell lung cancer cell line, HCT‑116 human colorectal carcinoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EGFR (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EGFR (aa 746-750 deletion) at approximately 170 kDa (as indicated). For additional reference total EGFR was detected using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF231). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|EGFR (aa 746-750 deletion) in HCC827 Human Cell Line. EGFR (aa 746-750 deletion) was detected in immersion fixed HCC827 human non-small cell lung cancer cell line using Mouse Anti-Human EGFR (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. Negative staining shown in the lower panel with NCI-H1975 human lung epithelial cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Detection of Human EGFR by Simple WesternTM. Simple Western lane view shows lysates of HCC827 human non-small cell lung cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for EGFR at approximately 200 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human EGFR (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336). This experiment was conducted under reducing conditions and using the|
66-440 kDa separation system.
Epidermal growth factor receptor (EGFR, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. EGFR exon 19 deletions are in-frame deletions occurring within
exon 19, which encodes part of the kinase domain. This mutation occurs
with a frequency of approximately 48% in EGFR mutant lung tumors. It affects the catalytic domain (amino acids 746-750), and is predominantly associated with non-small cell lung cancer (1). In a metastatic setting, EGFR deletions like aa746-750 are predictors of efficacy of the EGFR tyrosine kinase inhibitors (1, 2).