|Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immunoprecipitated from lysates (5 x 106 cells) of Jurkat human acute T cell leukemia and CTLL‑2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.|
|Cytochrome c in Human PBMCs. Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
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