Human/Equine Cytochrome c Antibody
R&D Systems | Catalog # MAB898
Key Product Details
Species Reactivity
Validated:
Human, Equine
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 2B5F8
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Product Specifications
Immunogen
Equine Cytochrome c
Specificity
Detects native human Cytochrome c but not mouse Cytochrome c.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Equine Cytochrome c Antibody
Immunoprecipitation of Human/Mouse Cytochrome c.
Cytochrome c was immunoprecipitated from lysates (5 x 106cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complex-es were absorbed using Protein A Immunoprecipitin (Life Tech-nologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.
Cytochrome c in Human PBMCs.
Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Equine Cytochrome c Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (basal ganglia)
Sample: Immersion fixed paraffin-embedded sections of human brain (basal ganglia)
Immunoprecipitation
1 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line and CTLL‑2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Sample: Jurkat human acute T cell leukemia cell line and CTLL‑2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cytochrome c
Additional Cytochrome c Products
Product Documents for Human/Equine Cytochrome c Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Equine Cytochrome c Antibody
For research use only
Related Research Areas
Citations for Human/Equine Cytochrome c Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling: Oxidative Stress Pathway
