Human ErbB2/Her2 Herstatin Isoform Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ErbB2/Her2 Herstatin Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) EGFR or rhErbB3 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 416711
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ErbB2/Her2 Herstatin Isoform
    Thr23-Gly419
    Accession # AAD56009
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
ErbB2/Her2 in Human Breast. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 25 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB2/Her2
Herstatin is a secreted 68 kDa glycoprotein encoded by an alternate transcript of the HER-2/neu (erbB-2) gene containing the N-terminal 304 amino acids (aa) of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress HER-2.
  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB2
  • Entrez Gene IDs:
    2064 (Human); 13866 (Mouse); 24337 (Rat)
  • Alternate Names:
    CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
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