Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 182803
View all ErbB4/Her4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ErbB4/Her4
Gln26-Arg649
Accession # Q15303

Specificity

Detects human ErbB4/Her4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) EGF R, rhErbB2, and rhErbB3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ErbB4/Her4 Antibody

ErbB4/Her4 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

ErbB4/Her4 in MCF‑7 Human Cell Line.

ErbB4/Her4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB1131) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ErbB4/Her4 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

ErbB4/Her4 in Human Colon Cancer Tissue.

ErbB4/Her4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB1131) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ErbB4/Her4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human ErbB4/Her4 Fc Chimera (Catalog # 1131-ER)

Reviewed Applications

Read 2 reviews rated 5 using MAB1131 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB4/Her4

ErbB4, also called Her4 (human epidermal growth factor receptor 4), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has been found in ErbB4 and ErbB1 (EGFR). Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25 aa signal sequence, a 626 aa extracellular domain, a 24 aa transmembrane region, and a 633 aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.

References

  1. Plowman, G.D. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1746. 
  2. Elenius, K. et al. (1997) J. Biol. Chem. 272:26761.
  3. Elenius, K. et al. (1999) Oncogene 18:2607.
  4. Rio, C. et al. (2000) J. Biol. Chem. 275:10379.
  5. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  6. Sundaresan, S. et al. (1998) Endocrinology 139:4756.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB4

Alternate Names

HER4

Entrez Gene IDs

2066 (Human); 13869 (Mouse); 59323 (Rat)

Gene Symbol

ERBB4

UniProt

Q15303

Additional ErbB4/Her4 Products

Product Documents for Human ErbB4/Her4 Antibody

Certificate of Analysis

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Product Specific Notices for Human ErbB4/Her4 Antibody

For research use only

Citations for Human ErbB4/Her4 Antibody

Customer Reviews for Human ErbB4/Her4 Antibody (2)

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  • Human ErbB4/Her4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/20/2022
    Human ErbB4/Her4 Antibody MAB1131
  • Human ErbB4/Her4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 10/22/2021
    Human ErbB4/Her4 Antibody MAB1131

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