Human FAM3C Antibody

Catalog # Availability Size / Price Qty
MAB3084
MAB3084-SP

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

FAM3C in A549 Human Cell Line.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human FAM3C Antibody Summary

Species Reactivity
Human
Specificity
Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, recombinant mouse (rm) FAM3C, or rmFAM3D is observed.
Source
Monoclonal Mouse IgG2B Clone # 438618
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FAM3C
Gln25-Asp227
Accession # Q92520
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FAM3C
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry FAM3C antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

FAM3C in A549 Human Cell Line. FAM3C was detected in immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B) for 48 hours using Mouse Anti-Human FAM3C Monoclonal Antibody (Catalog # MAB3084) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM3C

FAM3C (Family with sequence similarity 3, member C), also known as D6WSU176e, is a secreted protein belonging to a cytokine-like family. Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. FAM3C is ubiquitously expressed in multiple tissues. The human FAM3C gene has been identified to be a candidate gene for autosomal recessive non-syndromic hearing loss locus 17. The amino acid sequence of mature mouse FAM3C is 97% and 94% identical to that of rat and human FAM3C, respectively.

Long Name
Family With Sequence Similarity 3, Member C
Entrez Gene IDs
10447 (Human); 27999 (Mouse); 312159 (Rat)
Alternate Names
D6WSU176e; FAM3C; family with sequence similarity 3, member C; GS3876; ILEI; ILEIInterleukin-like EMT inducer; predicted osteoblast protein

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human FAM3C Antibody

There are currently no reviews for this product. Be the first to review Human FAM3C Antibody and earn rewards!

Have you used Human FAM3C Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review