Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, recombinant mouse (rm) FAM3C, or rmFAM3D is observed.
Monoclonal Mouse IgG2B Clone # 438618
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human FAM3C Gln25-Asp227 Accession # Q92520
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
FAM3C in A549 Human Cell Line.
FAM3C was detected in immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B) for 48 hours using Mouse Anti-Human FAM3C Monoclonal Antibody (Catalog # MAB3084) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FAM3C (Family with sequence similarity 3, member C), also known as D6WSU176e, is a secreted protein belonging to a cytokine-like family. Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. FAM3C is ubiquitously expressed in multiple tissues. The human FAM3C gene has been identified to be a candidate gene for autosomal recessive non-syndromic hearing loss locus 17. The amino acid sequence of mature mouse FAM3C is 97% and 94% identical to that of rat and human FAM3C, respectively.
Family With Sequence Similarity 3, Member C
Entrez Gene IDs:
10447 (Human); 27999 (Mouse); 312159 (Rat)
D6WSU176e; FAM3C; family with sequence similarity 3, member C; GS3876; ILEI; ILEIInterleukin-like EMT inducer; predicted osteoblast protein
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