Key Product Details

Species Reactivity

Validated:

Human

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGF-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-10
Cys37-Ser208
Accession # O15520

Specificity

Detects human FGF-10 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human FGF‑10 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF‑10 Monoclonal Antibody (Catalog # MAB3451).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF‑10 (Catalog # 345-FG)

Reviewed Applications

Read 3 reviews rated 4 using AF345 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-10

The FGFs are a growing family of heparin-binding growth factors that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share significant amino acid sequence homology. FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse FGF-10 were subsequently cloned. The human FGF-10 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human FGF-10 shares approximately 92% and 95% amino acid sequence identity with mouse and rat
FGF‑10, respectively. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

References

  1. Yamasaki, M. et al. (1996) J. Biol. Chem. 271:15918.
  2. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  3. Ohuchi, H. et al. (1997) Development 124:2235.
  4. Tagashira, S. et al. (1997) Gene 197:399.
  5. Bellusci, S. et al. (1997) Development 124:4867.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

UniProt

O15520

Additional FGF-10 Products

Product Documents for Human FGF‑10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FGF‑10 Antibody

For research use only

Related Research Areas

FGF Family
FGF Ligands
gamma delta T Cells

Citations for Human FGF‑10 Antibody

Customer Reviews for Human FGF‑10 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • FGF10 IF staining in mouse lungs
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    IF staining of FGF10 in mouse lungs (green) 10x
    FGF10 treated mice lungs staining
    Human FGF‑10 Antibody AF345
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human FGF-10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate cancer tissue
    Species: Human
    Verified Customer | Posted 10/05/2021
    Human FGF‑10 Antibody AF345
  • Human FGF-10 Antibody
    Name: Preethi Vijayaraj
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPSC derived epithelial cells
    Species: Human
    Verified Customer | Posted 09/27/2017
    Human FGF‑10 Antibody AF345

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Protocols

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FAQs

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