Key Product Details

Species Reactivity

Validated:

Human

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGF-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-10
Cys37-Ser208
Accession # O15520

Specificity

Detects human FGF-10 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human FGF-10 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF‑10 Monoclonal Antibody (Catalog # MAB3451).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF‑10 (Catalog # 345-FG)

Reviewed Applications

Read 3 reviews rated 4 using AF345 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-10

The FGFs are a growing family of heparin-binding growth factors that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share significant amino acid sequence homology. FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse FGF-10 were subsequently cloned. The human FGF-10 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human FGF-10 shares approximately 92% and 95% amino acid sequence identity with mouse and rat
FGF‑10, respectively. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

References

  1. Yamasaki, M. et al. (1996) J. Biol. Chem. 271:15918.
  2. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  3. Ohuchi, H. et al. (1997) Development 124:2235.
  4. Tagashira, S. et al. (1997) Gene 197:399.
  5. Bellusci, S. et al. (1997) Development 124:4867.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

UniProt

O15520

Additional FGF-10 Products

Product Documents for Human FGF-10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FGF-10 Antibody

For research use only

Related Research Areas

FGF Family
FGF Ligands
gamma delta T Cells

Citations for Human FGF-10 Antibody

Customer Reviews for Human FGF-10 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • FGF10 IF staining in mouse lungs
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    IF staining of FGF10 in mouse lungs (green) 10x
    FGF10 treated mice lungs staining
    Human FGF-10 Antibody AF345
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human FGF-10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate cancer tissue
    Species: Human
    Verified Customer | Posted 10/05/2021
    Human FGF-10 Antibody AF345
  • Human FGF-10 Antibody
    Name: Preethi Vijayaraj
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPSC derived epithelial cells
    Species: Human
    Verified Customer | Posted 09/27/2017
    Human FGF-10 Antibody AF345

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Protocols

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FAQs

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