Detects human FGL2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-like 2, and recombinant mouse Angiopoietin-3 is observed.
Monoclonal Mouse IgG2B Clone # 532223
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human FGL2 Asn24-Pro439 Accession # Q14314
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FGL2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FGL2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FGL2 in WI‑38 Human Cell Line. FGL2 was detected in immersion fixed WI‑38 human fetal lung fibroblast cell line using Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64‑70 kDa secreted glycoprotein of the fibrinogen-like superfamily. It is produced by macrophages and endothelial cells in response to inflammatory mediators, T cells and fetal trophoblast cells. FGL2 has prothrombinase activity, promotes immune-mediated thrombosis and functions in transplant rejection and infection-related abortion. Mature human FGL2 gene is a 416 amino acid (aa) protein with a coiled-coil region and a fibronectin C-terminal homology domain or FRED. Coiled-coil dimers are covalently linked to form a tetrameric 260‑280 aa FGL2 complex. Mature human FGL2 shares 79% aa identity with mouse FGL2.
Entrez Gene IDs:
10875 (Human); 14190 (Mouse); 84586 (Rat)
FGL2; fibrinogen-like 2; Fibrinogen-like protein 2; Fibroleukin; pT49T49; T49
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